Phospho-Tau-T181 Rabbit Polyclonal Antibody (WB, IF, ELISA) - P10636

Core Product Specifications and Parameters

Biological Background: Tau Function and Localization

• Tau is a microtubule-associated protein that promotes microtubule assembly and stability, playing a critical role in establishing and maintaining neuronal polarity. Related references: PMID:21985311
• The C-terminal region binds axonal microtubules while the N-terminus interacts with neural plasma membrane components, suggesting a linker function between both. Related references: PMID:21985311 PMID:32961270
• Alternative splicing generates six brain isoforms; short isoforms allow cytoskeletal plasticity while longer isoforms preferentially play a role in stabilization.
• Subcellular localization includes cytoplasm, cytosol, cell membrane, cytoskeleton, axons, dendrites, and secreted forms.
• Highly expressed in neurons; isoform PNS-tau is primarily in the peripheral nervous system, while others are expressed in the central nervous system.
• Hyperphosphorylation, including at T181, leads to paired helical filament formation, a hallmark of Alzheimer disease and other tauopathies.
• Undergoes multiple post-translational modifications including phosphorylation, acetylation, methylation, glycation, and ubiquitination, which regulate its function and aggregation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide; to confirm specificity for T181 phosphorylation, it is advisable to use phospho-deficient and phospho-mimic controls in parallel assays.
• For Western blot, a major band around 79 kDa is expected; additional bands corresponding to splice variants or phosphorylation states may appear depending on sample preparation.
• For immunofluorescence/ICC, optimize permeabilization and blocking conditions; co-staining with a total Tau antibody can help verify phosphorylation-specific localization.
• Cross-reactivity with mouse and rat allows application in rodent tauopathy models; consider species-specific validation in your experimental system.
• ELISA applications may benefit from optimization using known phospho-peptide standards as calibrators.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibodies like this phospho-Tau-T181 antibody for neurodegenerative disease research. Our portfolio includes bulk reagents, ancillary materials, and rare target sourcing to support diagnostic development from concept to clinic. Contact us to secure reliable supply for your tauopathy assay needs.