Phospho-Tau-T181 Rabbit pAb for WB and ELISA - P10636-5

Core Product Specifications and Parameters

Biological Background: Microtubule-associated protein tau (Isoform Tau-C) Function and Localization

• The target is isoform Tau-C of microtubule-associated protein tau, encoded by the MAPT gene (also known as MAPTL, MTBT1, TAU).
• Alternative names include Neurofibrillary tangle protein and Paired helical filament-tau (PHF-tau), reflecting its identification in pathological aggregates characteristic of Alzheimer's disease and other tauopathies.
• As a microtubule-associated protein, tau is predominantly expressed in neurons and is involved in microtubule stabilization, although detailed functional annotations for isoform Tau-C are not provided in the current record.
• The calculated molecular weight of isoform Tau-C is approximately 42.6 kDa, but experimentally observed bands often range from 32–45 kDa and 78–80 kDa due to alternative splicing, phosphorylation, and other post-translational modifications.
• Phosphorylation at threonine 181 (T181) is a well-known pathological modification associated with hyperphosphorylated tau conformations and paired helical filament formation.
• The immunogen design targets this specific phosphorylation site, enabling selective detection of T181‑phosphorylated tau.

Experimental Guidance and Technical Tips

• For Western blot analysis, consider using positive controls such as brain tissue lysates from transgenic Alzheimer's disease mouse models or human neuroblastoma cell lines treated with phosphatase inhibitors.
• Validate phosphorylation specificity by comparing signals before and after phosphatase treatment of samples or by using a non‑phospho‑specific tau antibody in parallel.
• In ELISA applications, the antibody may be suitable for detecting phosphorylated tau in cerebrospinal fluid or plasma as a potential biomarker for Alzheimer's disease; optimization of coating and blocking conditions is recommended.
• Owing to the multiple tau isoforms and extensive post‑translational modifications, expect multiple bands or diffuse signals in WB; run appropriate molecular weight markers and confirm by mass spectrometry if possible.
• Ensure samples are kept at low temperatures and use protease/phosphatase inhibitors throughout preparation to preserve phosphorylation status.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic and research laboratories with comprehensive access to IVD raw materials and technical expertise, streamlining the journey from concept to clinical application. We provide this high‑quality phospho‑tau antibody alongside a broad portfolio of validated antibodies, matched antibody pairs, and ancillary reagents for neurodegenerative disease research. Our sourcing capabilities cover rare targets and custom formulations, making CamelBio a reliable partner for your tauopathy assay development needs.