Phospho-Tau-T181 Rabbit mAb for WB, ELISA - P10636

Core Product Specifications and Parameters

Biological Background: Tau Function and Localization

• Microtubule-associated protein tau (MAPT) is primarily expressed in neurons and plays a critical role in promoting microtubule assembly and stability. Related references: PMID:21985311, PMID:32961270
• Tau functions as a linker protein: its C-terminus binds axonal microtubules while its N-terminus associates with neural plasma membrane components, contributing to neuronal polarity. Related references: PMID:21985311, PMID:32961270
• The MAPT gene encodes multiple isoforms via alternative splicing; shorter isoforms promote cytoskeletal plasticity, whereas longer isoforms likely stabilize microtubules.
• In neurons, tau localizes to the cytoplasm, cytoskeleton, axons, dendrites, and can be secreted; its subcellular distribution is critical for neuronal function.
• Tissue-specific expression includes central nervous system isoforms and a peripheral nervous system-specific isoform (PNS-tau).
• Post-translational modifications such as phosphorylation (e.g., at T181), acetylation, methylation, and ubiquitination regulate tau function and are implicated in neurodegenerative diseases.
• Aberrant hyperphosphorylation of tau leads to paired helical filament (PHF) formation, a hallmark of Alzheimer disease and other tauopathies.

Experimental Guidance and Technical Tips

• For Western blot, consider using human neuronal cell lysates or brain tissue extracts; validate phospho-specificity by treating samples with lambda phosphatase to confirm loss of signal at the expected molecular weight (~79 kDa).
• In ELISA applications, pair this capture/detection antibody with a non-phospho Tau antibody to quantify site-specific phosphorylation levels.
• As the antibody is validated for human Tau only, cross-reactivity with other species should be verified experimentally if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw-material sourcing, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For Tau-targeted neurodegenerative disease research, we provide tailored solutions from concept to clinic, leveraging our expertise to streamline your diagnostic development.