Phospho-Tau-Ser404 Rabbit pAb - P10636

Core Product Specifications and Parameters

Biological Background: Tau Function and Localization

• Tau is a microtubule-associated protein that promotes microtubule assembly and stability, playing a critical role in establishing and maintaining neuronal polarity. Related references: PMID:21985311, PMID:32961270
• It functions as a linker between axonal microtubules (via its C-terminus) and neural plasma membrane components (via its N-terminus), contributing to axonal organization. Related references: PMID:21985311, PMID:32961270
• Multiple isoforms are generated by alternative splicing, with shorter isoforms allowing cytoskeletal plasticity and longer isoforms stabilizing microtubules.
• It is predominantly expressed in neurons, with CNS-specific isoforms and the PNS-tau isoform found in the peripheral nervous system.
• Subcellularly, tau localizes to the cytoplasm, cytoskeleton, cell membrane, and is enriched in axons and dendrites; it can also be secreted.
• Heavily phosphorylated under pathological conditions; hyperphosphorylation leads to aggregation into paired helical filaments (PHFs) and neurofibrillary tangles, hallmarks of Alzheimer's disease and other tauopathies.
• Post-translational modifications include acetylation, methylation, glycation, ubiquitination, and disulfide bond formation, regulating its function and aggregation propensity.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around Ser404 of human tau, suggesting the antibody recognizes the phosphorylation site. Validate specificity using phosphatase-treated samples or site-directed mutants.
• Suitable for WB, IF-P, and ELISA per supplier specifications; consider optimizing dilution and detection conditions for your specific experimental system.
• Cross-reacts with human, mouse, and rat tau; confirm reactivity in your samples by including appropriate positive and negative controls.
• For immunofluorescence, ensure proper permeabilization and fixation protocols to preserve phospho-epitopes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-Phospho-Tau-Ser404 antibody supports research into Alzheimer's disease, tauopathies, and neurodegeneration. Beyond this reagent, CamelBio can source validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target raw materials to streamline your assay development and supply chain.