Phospho-Tau-S262 Rabbit pAb - P10636

Core Product Specifications and Parameters

Biological Background: Tau Function and Localization

• Microtubule-associated protein tau (MAPT) promotes microtubule assembly and stability, and is crucial for neuronal polarity. The C-terminal domain binds microtubules, while the N-terminal domain interacts with the plasma membrane, positioning tau as a linker protein. Related references: PMID:21985311 PMID:32961270
• Alternative splicing of the MAPT gene generates multiple isoforms; short isoforms confer cytoskeletal plasticity, whereas long isoforms stabilize microtubules.
• Tau localizes to the cytoplasm, cytosol, cell membrane, cytoskeleton, axonal and dendritic projections, and can be secreted.
• Expressed predominantly in neurons; the PNS-tau isoform is expressed in the peripheral nervous system, while others are in the central nervous system.
• Undergoes extensive post-translational modifications, including phosphorylation (e.g., at S262), acetylation, methylation, glycation, ubiquitination, and disulfide bond formation.
• Aggregation of hyperphosphorylated tau into neurofibrillary tangles is a hallmark of Alzheimer disease and related tauopathies.

Experimental Guidance and Technical Tips

• For Western blot, use the antibody to detect phosphorylated Tau (S262). A total Tau antibody can be used in parallel to normalize phosphorylation levels.
• In immunofluorescence/immunocytochemistry (IF/ICC) and immunofluorescence on paraffin sections (IF-P), ensure proper fixation and permeabilization to preserve phospho-epitopes.
• For ELISA, the antibody may serve as a capture or detection reagent following appropriate optimization.
• Verify specificity using phosphatase-treated samples or blocking peptides to confirm phospho-dependent binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, offering a comprehensive portfolio of validated antibodies, recombinant proteins, and ancillary reagents. This phospho-Tau-S262 antibody supports Tau-related neurodegeneration research and assay development. From antibody pairs to bulk reagents and rare-target sourcing, CamelBio streamlines procurement for diagnostic manufacturers and research institutes.