Phospho-Tau (S214) Rabbit mAb for WB, IHC-P, ELISA - P10636

Core Product Specifications and Parameters

Biological Background: Microtubule-associated Protein Tau Function and Localization

• Microtubule-associated protein tau (MAPT) is a neuronal phosphoprotein that stabilizes and promotes the assembly of microtubules, particularly in axons, and acts as a linker between microtubules and the plasma membrane through its C-terminus and N-terminus, respectively. Related references: PMID:21985311 PMID:32961270
• Multiple tau isoforms are generated by alternative splicing, with shorter isoforms allowing cytoskeletal plasticity and longer isoforms preferentially stabilizing microtubules in the central nervous system.
• Tau localizes to the cytoplasm, cytosol, cytoskeleton, axons, dendrites, and cell membrane, and can also be secreted extracellularly.
• Expressed predominantly in neurons, with isoform PNS-tau specific to the peripheral nervous system and other isoforms in the central nervous system.
• Post-translational modifications include phosphorylation (e.g., at S214), acetylation, methylation, glycation, and ubiquitination, with phosphorylation regulating microtubule binding and aggregation.
• Mutations in MAPT are associated with neurodegenerative tauopathies such as Alzheimer's disease, frontotemporal dementia with parkinsonism-17 (FTDP-17), and Pick's disease.

Experimental Guidance and Technical Tips

• For Western blot, consider using phosphatase inhibitors in lysis buffers and validate the detected band (~79 kDa) in relevant neuronal cell lines or brain tissues.
• In immunohistochemistry (IHC-P), antigen retrieval and phospho-specific detection protocols may need optimization; include appropriate controls (e.g., phosphatase-treated sections).
• The antibody cross-reacts with mouse, enabling translational studies in mouse models; confirm specificity in the appropriate sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research teams with one-stop access to IVD raw materials for neurodegenerative disease targets like tau. We provide validated rabbit monoclonal antibodies, ready-to-use bulk ancillary reagents, and sourcing capabilities for rare phospho-specific and isoform-specific reagents to accelerate your assay development.