Phospho-TAK1 (S412) Rabbit Polyclonal Antibody - O43318

Core Product Specifications and Parameters

Biological Background: TAK1 Function and Localization

• Acts as an essential serine/threonine kinase in the MAP kinase signal transduction pathway, mediating cellular responses to environmental changes. Related references: PMID:10094049, PMID:11460167, PMID:12589052
• Mediates signal transduction from various receptors including IL-1, TGF-β, TLR, CD40, and BCR, linking them to downstream pathways. Related references: PMID:16893890, PMID:9079627
• Phosphorylates and activates MAP2K kinases (MEK1, MKK3/6/7), leading to activation of JNK and p38 MAPK cascades and transcription factor AP-1. Related references: PMID:11460167, PMID:8663074, PMID:12589052
• Functions as a key activator of NF-κB by promoting IKK complex activation, often involving recruitment via TAB2/TAB3 to polyubiquitin chains. Related references: PMID:12589052, PMID:8663074, PMID:10094049, PMID:11460167
• Subcellular localization includes cytoplasm and cell membrane, with potential membrane recruitment upon activation.
• Tissue-specific isoform expression: isoform 1A abundant in ovary, skeletal muscle, spleen and blood mononuclear cells; isoform 1B in brain, kidney and small intestine; isoform 1C in prostate; isoform 1D less abundant.
• Phosphorylation at Ser412, among other residues, modulates TAK1 kinase activity and downstream signaling, consistent with its designation as a phosphoprotein.

Experimental Guidance and Technical Tips

• Consider using phosphatase inhibitors during sample preparation to preserve phosphorylation signals when performing Western blot.
• Validate phospho‑specificity by comparing signals with non‑phospho peptide competition or phosphatase‑treated lysates.
• Suitable for detecting TAK1 activation in human, mouse, and rat samples following stimulation with cytokines or TLR agonists.
• For ELISA, ensure proper coating and detection conditions for phosphorylated targets.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one‑stop partner for IVD raw‑material sourcing, providing high‑quality reagents such as this anti‑Phospho‑TAK1 (S412) antibody for MAP kinase and NF‑κB pathway research. Our extensive portfolio includes validated phospho‑specific antibodies, bulk ancillary reagents, and custom sourcing solutions to accelerate your diagnostic development projects.