Phospho-Syk-Y323 Rabbit pAb for WB, IF/ICC - P43405

Core Product Specifications and Parameters

Biological Background: Tyrosine-protein Kinase SYK Function and Localization

• Tyrosine-protein kinase SYK (spleen tyrosine kinase, p72-Syk) is a non-receptor tyrosine kinase that mediates signal transduction downstream of a variety of transmembrane receptors, including classical immunoreceptors such as the B-cell receptor (BCR). Related references: PMID:12456653, PMID:15388330, PMID:34634301
• Via its twin SH2 domains, SYK assembles into signaling complexes with activated receptors at the plasma membrane by binding to tyrosine-phosphorylated ITAM domains, a process often initiated by SRC family kinases.
• In B-cells, SYK is essential for maturation at the pro-B to pre-B transition and phosphorylates key adapters like BLNK, as well as PLCG1 and BTK, to propagate BCR signaling. Related references: PMID:12456653
• SYK plays a crucial role in innate immunity by mediating responses to fungal, bacterial, and viral pathogens, for example via CLEC7A, leading to ROS production and inflammasome activation.
• Beyond immunoreceptors, SYK functions downstream of cell adhesion receptors, relaying integrin-mediated activation of neutrophils and macrophages, and is involved in platelet activation by collagen and podoplanin. Related references: PMID:12387735
• SYK regulates diverse biological processes including osteoclast maturation, vascular development, and the intestinal immune response via NF-κB-mediated cytokine production. Related references: PMID:12387735, PMID:33782605
• Subcellularly, SYK is found at the cell membrane and in the cytoplasm/cytosol. It is widely expressed in hematopoietic cells, particularly in neutrophils and throughout B-cell development.
• Post-translational modifications include phosphorylation (the Y323 site is critical for activation), and it contains SH2 domains and an ATP-binding kinase domain.

Experimental Guidance and Technical Tips

• This antibody was raised against a phospho-specific peptide surrounding tyrosine 323 of human SYK. The immunogen sequence corresponds to a phosphorylation site known to regulate SYK activation and downstream signaling.
• For optimal detection of phosphorylated SYK, include phosphatase inhibitors in all lysis and assay buffers to preserve the phosphorylation state of the target protein.
• The product is recommended for WB and IF/ICC. In WB, a band near 72 kDa is expected. For IF/ICC, fixation and permeabilization steps should be optimized for the target’s membrane and cytosolic localizations.
• Consider validating the antibody in your relevant sample system under conditions where SYK phosphorylation is induced (e.g., BCR stimulation) versus non-induced controls to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers this phospho-specific SYK (Y323) rabbit polyclonal antibody as part of a comprehensive catalog of IVD-grade raw materials tailored for immunology and cell signaling research. Whether you need validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, or assistance sourcing rare target raw materials, our team bridges the gap from concept to clinic, empowering diagnostic manufacturers and laboratories with reliable supply solutions.