Phospho-STK11-S334 Rabbit Polyclonal Antibody for WB, ELISA - Q15831

Core Product Specifications and Parameters

Biological Background: STK11 (LKB1) Function and Localization

• STK11 (also known as LKB1 or hLKB1) is a tumor suppressor serine/threonine-protein kinase that controls AMPK family members, playing critical roles in cell metabolism, polarity, apoptosis, and DNA damage response. Related references: PMID:12805220, PMID:14517248, PMID:14976552
• It phosphorylates the T-loop of AMPK family proteins, including PRKAA1, PRKAA2, BRSK1, and MARK kinases, thereby activating them and regulating downstream metabolic and polarity pathways.
• It also phosphorylates non-AMPK family proteins such as STRADA, PTEN, and possibly p53/TP53, linking its activity to broader signaling networks. Related references: PMID:11430832, PMID:15987703
• STK11 functions as a key upstream regulator of AMPK, mediating responses to energy stress, glucose homeostasis in liver, autophagy induction, and B-cell differentiation in germinal centers. Related references: PMID:14976552
• The kinase is involved in DNA damage response by interacting with p53 and regulating CDKN1A/WAF1 transcription, and it translocates to mitochondria during apoptosis. Related references: PMID:11430832, PMID:17108107, PMID:21317932
• It contributes to UV radiation-induced DNA repair by phosphorylating CDKN1A in association with NUAK1, promoting CDKN1A degradation for optimal repair. Related references: PMID:25329316
• Subcellularly, STK11 is found in the nucleus, cytoplasm, membrane, mitochondrion, and late endosomes, consistent with its diverse regulatory roles.
• It is ubiquitously expressed with strongest levels in testis and fetal liver, and has a specific role in spermiogenesis.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic phosphorylated peptide around Ser334 of human STK11; therefore, it specifically recognizes the phosphorylated form at this residue.
• For Western blot, include phosphatase inhibitors during sample preparation to preserve phosphorylation. Consider stimulating cells with agents that activate STK11/LKB1 pathways (e.g., energy stress) to enhance signal.
• Validate antibody specificity using phosphatase treatment or competition with phosphorylated vs. non-phosphorylated peptides.
• Since cross-reactivity is reported with mouse and rat, the antibody may be used for orthologous protein detection; however, confirm alignment of the Ser334 site across species.
• For ELISA, use appropriate coating and detection protocols with phospho-specific controls. Optimize antibody dilutions empirically for your assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your one-stop bridge for sourcing high-quality IVD raw materials, including this anti-phospho-STK11 antibody for tumor suppressor and metabolic research. We supply validated antibody pairs, bulk monoclonal/polyclonal antibodies, and ancillary reagents to streamline diagnostic development from concept to clinic. Our team also supports rare target sourcing and technical consulting to meet your specific project needs.