Phospho-STING/TMEM173-S366 Rabbit pAb for WB and ELISA - Q86WV6

Core Product Specifications and Parameters

Biological Background: STING/TMEM173 Function and Localization

• Stimulator of interferon genes protein (STING/TMEM173), also referred to as ERIS, MITA, MPYS, and hSTING, is encoded by the STING1 gene in humans and exists as multiple isoforms including STING-beta.
• Acts as a cytosolic DNA sensor and facilitates innate immune signaling, promoting type I interferon (IFN-α/β) production upon recognition of non-CpG double-stranded DNA from viruses and bacteria. Related references: PMID:18724357 PMID:18818105 PMID:19433799
• Binds cyclic dinucleotides such as bacterial c-di-GMP and endogenous cGAMP (2',3'-cGAMP) produced by cGAS, triggering its oligomerization and translocation from the endoplasmic reticulum to the Golgi. Related references: PMID:21947006 PMID:23258412 PMID:23707065
• Phosphorylation at Ser366 (located in the pLxIS motif) by TBK1 is essential for recruitment and activation of IRF3, leading to interferon expression. Related references: PMID:22394562 PMID:25636800 PMID:29973723
• Beyond interferon induction, STING directly mediates autophagy by budding from the ER in COPII vesicles and forming ERGIC membranes that recruit WIPI2 and LC3, facilitating degradation of cytosolic DNA. Related references: PMID:30568238 PMID:30842662
• Ubiquitously expressed, with notable presence in skin endothelial cells, alveolar type 2 pneumocytes, bronchial epithelium, and alveolar macrophages. Related references: PMID:18724357 PMID:18818105 PMID:25029335
• Subcellular localization spans the endoplasmic reticulum, ERGIC, Golgi apparatus, mitochondrial outer membrane, autophagosome membrane, and possibly the plasma membrane.

Experimental Guidance and Technical Tips

• Since the immunogen is a synthetic phosphorylated peptide around Ser366, this antibody is expected to specifically recognize STING/TMEM173 only when phosphorylated at this site, which occurs upon activation of the cGAS-STING pathway (e.g., after dsDNA or cGAMP stimulation).
• For Western blotting (WB), phosphorylated STING can be detected in human cell lysates following treatment with cGAMP or dsDNA to stimulate the pathway; a band at approximately 42 kDa is expected. Non-phosphorylated STING should not be detected.
• In ELISA applications, this antibody can be used to capture or detect phosphorylated STING in cell or tissue extracts; consider pairing with a total STING antibody for normalization or sandwich assays.
• Always validate the antibody's performance in your specific experimental system, as phosphorylation levels may vary with cell type and stimulation conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and clinical development teams with streamlined access to high-quality IVD raw materials. For STING/TMEM173 research, we offer validated phospho-specific and total antibodies, optimized antibody pairs for ELISA development, and bulk ancillary reagents to support innate immunity and immuno-oncology applications. As your sourcing bridge, CamelBio also facilitates procurement of rare-target raw materials and custom reagent development from concept to clinic.