Phospho-STAT6-Y641 Rabbit pAb - P42226

Core Product Specifications and Parameters

Biological Background: STAT6 Function and Localization

• STAT6 is a dual-function protein that transduces signals from IL-4 and IL-3 receptors and activates transcription of target genes.
• Upon activation, STAT6 translocates from the cytoplasm to the nucleus to regulate gene expression.
• The protein contains a central SH2 domain that mediates its interaction with phosphorylated tyrosine residues on activated receptors.
• Key post-translational modifications include phosphorylation at Y641 (critical for dimerization and activity), acetylation, and ADP-ribosylation.
• Multiple isoforms (e.g., STAT6B, STAT6C) are produced by alternative splicing, and variants have been associated with disease.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide around Y641; thus, the antibody may react with non-phosphorylated STAT6 unless specifically validated. Use appropriate controls.
• For Western blot, include phosphatase inhibitors in lysis buffer to maintain phosphorylation. Consider treating samples with lambda phosphatase to confirm phospho-specificity.
• IHC-P may require antigen retrieval and careful optimization of detection conditions; confirm staining with appropriate positive and negative tissue controls.
• The antibody is supplied as a rabbit polyclonal; note that lot-to-lot consistency can vary, so we recommend performing validation experiments for each new lot.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, supporting diagnostic manufacturers and research labs with validated antibodies, bulk reagents, and custom sourcing. Our Phospho-STAT6-Y641 antibody is ideal for IL-4/STAT6 pathway investigations, and we can assist with ancillary reagents, technical services, and rare target procurement to streamline your R&D from concept to clinic. Contact our B2B team for competitive quotes and tailored supply solutions.