Phospho-STAT5A-Y694 Rabbit pAb for WB, ELISA - P42229

Core Product Specifications and Parameters

Biological Background: STAT5A Function and Localization

• Signal transducer and activator of transcription 5A (STAT5A) is a member of the STAT protein family, characterized by an SH2 domain that mediates phosphorylation-dependent dimerization.
• It functions as both a cytoplasmic signal transducer and a nuclear transcription activator upon cytokine or growth factor stimulation.
• Mediates cellular responses to KITLG/SCF, ERBB4, and activated FGFR1-4, among other signals.
• In the nucleus, STAT5A binds to the GAS element and activates prolactin (PRL)-induced transcription, regulating expression of milk proteins during lactation.
• The protein localizes to both cytoplasm and nucleus, shuttling in response to activation signals.
• Post-translational modifications include phosphorylation, notably at Y694, which is critical for dimerization and transcriptional activity.
• Alternative splicing produces multiple isoforms; the canonical sequence has a molecular weight of approximately 90.6 kDa.
• Keywords: Phosphoprotein, DNA-binding, SH2 domain, transcription regulation, lactation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around tyrosine 694, designed to specifically recognize the activated, phosphorylated form of STAT5A. For total STAT5A detection, a non-phospho control antibody may be considered.
• The antibody is suggested for Western blot (WB) and ELISA applications. Optimal dilutions and conditions should be empirically determined in the relevant sample system.
• Because STAT5A shuttles between cytoplasm and nucleus upon activation, consider appropriate cell fractionation or lysis buffers to preserve the phosphorylation state and localization context.
• Cross-reactivity is indicated for human; reactivity with other species is not provided and should be verified experimentally if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, bulk ancillary reagents, and specialized sourcing for rare targets, supporting diagnostic manufacturers from concept to clinic. This phospho-specific STAT5A antibody is suited for research in cytokine signaling pathways, cancer biomarker discovery, and lactation biology. As a trusted one-stop partner, CamelBio streamlines your supply chain for reliable, high-quality reagents.