Phospho-STAT5A-Y694 Rabbit mAb for WB, IHC-P, ELISA - P42229

Core Product Specifications and Parameters

Biological Background: STAT5A Function and Localization

• STAT5A is a dual-function protein that acts in signal transduction and activation of transcription, mediating cellular responses to various cytokines and growth factors.
• It is a key downstream mediator for KITLG/SCF, ERBB4, and activated FGF receptors (FGFR1-4).
• STAT5A binds to the GAS element and promotes prolactin-induced transcription, essential for regulating milk protein gene expression during lactation.
• Upon activation, STAT5A translocates from the cytoplasm to the nucleus to exert its transcriptional regulatory functions.
• The protein undergoes post-translational modifications including phosphorylation (e.g., at Y694), ubiquitination, and alternative splicing, which modulate its activity.
• It features an SH2 domain for signal binding and a DNA-binding domain for gene regulation, classifying it as a transcription factor activator.
• STAT5A has been identified in proteomics studies and serves as a reference proteome in comprehensive human protein catalogs.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around Y694 of human STAT5A (UniProt P42229); ensure that sample preparation includes phosphatase inhibitors to preserve the phosphorylation state.
• For Western blot detection, consider treating cells with appropriate cytokines (e.g., prolactin, IL-2, or growth factors) to induce STAT5A phosphorylation before lysate collection.
• In immunohistochemistry (IHC-P), optimize antigen retrieval and blocking conditions, and validate phospho-specific staining using appropriate positive and negative control tissues.
• For ELISA applications, the peptide-competitive assay can be used to confirm signal specificity.
• As with any phospho-specific antibody, validate the antibody’s performance in your specific experimental system using known phosphorylated and non-phosphorylated protein samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic developers from concept to clinic. For STAT5A and related cell signaling research, we provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing of rare-target raw materials. Whether you need high-quality phospho-specific antibodies or full assay development solutions, CamelBio bridges your requirements with reliable supply and technical support.