Phospho-STAT3-Y705 Rabbit pAb for WB, IHC-P, ELISA - P40763

Core Product Specifications and Parameters

Biological Background: STAT3 Function and Localization

• STAT3 is a critical signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP, and other growth factors, with documented roles in IL-6, IL-31, and oncostatin-M signaling pathways. Related references: PMID:10688651, PMID:11294841, PMID:12359225, PMID:15194700, PMID:9188471
• Upon activation, STAT3 recruits coactivators such as NCOA1 or MED1 to target gene promoters, thereby regulating downstream transcription. Related references: PMID:15653507, PMID:16285960, PMID:17344214
• STAT3 plays a pivotal role in immune regulation by controlling the differentiation of naive CD4+ T-cells into Th17 or regulatory T-cells, a process modulated by acetylation and deacetylation/oxidation events. Related references: PMID:28065600, PMID:28262505
• In cell cycle regulation, STAT3 induces the expression of CCND1, facilitating the G1-to-S phase transition. Related references: PMID:17344214
• Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity, while under LEP stimulation it may promote apoptosis via BIRC5 transactivation. Related references: PMID:23084476, PMID:18242580
• STAT3 dynamically shuttles between the cytoplasm and nucleus; nuclear localization is essential for its transcriptional regulatory functions.
• STAT3 exhibits broad tissue expression, including heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas, and is found in naive CD4+ T cells as well as T-helper subsets. Related references: PMID:31899195
• Key post-translational modifications of STAT3 include phosphorylation at Y705 (critical for activation), acetylation, methylation, and ubiquitination-like modifications, consistent with its multifaceted regulation.

Experimental Guidance and Technical Tips

• This antibody is generated against a synthetic phospho-peptide corresponding to Y705 of human STAT3; it is expected to specifically recognize the tyrosine-705-phosphorylated form. Validate specificity using phosphatase treatment or phospho-deficient mutants in your experimental system.
• For Western blotting, the predicted band is at approximately 88 kDa. Additional bands may appear due to post-translational modifications or interacting proteins; appropriate controls are recommended.
• In IHC-P, optimize antigen retrieval and blocking steps. Phospho-specific detection conditions may enhance signal; consider phosphatase inhibitors during sample preparation.
• For ELISA applications, coating with the phospho-peptide immunogen or capturing phosphorylated STAT3 from cell lysates can be employed. Titration is advised to determine the optimal antibody concentration.
• Reported cross-reactivity with mouse and rat STAT3 enables multi-species studies; however, verify specificity in non-human samples, as sequence variations could influence binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a streamlined procurement pathway for IVD raw materials, connecting diagnostic manufacturers and research laboratories with validated phospho-specific antibodies like this anti-STAT3-Y705 product. Leveraging expertise in JAK/STAT signaling and immune-related targets, CamelBio can supply antibody pairs, bulk ancillary reagents, and support rare target sourcing to accelerate your assay development from concept to clinic.