Phospho-STAT3-Y705 Rabbit mAb for WB, IF-P, IHC-P, ELISA - P40763

Core Product Specifications and Parameters

Biological Background: STAT3 Function and Localization

• STAT3 (Signal transducer and activator of transcription 3) is a cytoplasmic transcription factor that mediates cellular responses to interleukins, KITLG/SCF, LEP, and other growth factors. Related references: PMID:10688651, PMID:11294841, PMID:12359225
• Upon activation, STAT3 translocates to the nucleus, recruits coactivators such as NCOA1 and MED1, and binds IL6-responsive elements to regulate acute-phase protein genes. Related references: PMID:15653507, PMID:16285960, PMID:17344214, PMID:12359225
• STAT3 mediates signaling via activated FGFRs and is involved in oncostatin-M pathways. Related references: PMID:12873986, PMID:9188471
• It plays a critical role in inflammatory responses, regulating T-helper cell differentiation (Th17/Treg) through acetylation and oxidation state. Related references: PMID:28065600, PMID:28262505
• Involved in cell cycle progression by inducing CCND1 expression and may promote apoptosis via BIRC5 transactivation under leptin signaling. Related references: PMID:17344214, PMID:18242580
• Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity. Related references: PMID:23084476
• STAT3 is widely expressed, including in heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, and immune cells such as naive CD4+ T cells and Th17/Th1/Th2 subsets.
• Key post-translational modifications include acetylation, phosphorylation, and methylation (Keywords: Acetylation, Phosphoprotein, Methylation).

Experimental Guidance and Technical Tips

• This rabbit monoclonal antibody is generated against a synthetic phosphorylated peptide around Y705 of human STAT3. Ensure sample preparation includes phosphatase inhibitors to preserve the phosphorylation state.
• For western blotting, a band around 88 kDa is expected. Optimize antibody dilution and use appropriate phospho-specific blocking reagents if non-specific binding occurs.
• For IHC-P and IF-P, antigen retrieval methods such as heat-induced epitope retrieval may be necessary. Include appropriate negative and positive controls.
• The antibody cross-reacts with mouse and rat STAT3 based on supplier validation; verify reactivity in your specific tissue or cell line when first used.
• Since it is a monoclonal antibody, it offers high lot-to-lot consistency, making it suitable for quantitative and comparative studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to premium IVD raw materials. This Phospho-STAT3-Y705 Rabbit mAb supports investigations into STAT3 phosphorylation signal transduction, inflammation, and oncology research. Our portfolio includes validated antibody pairs, bulk monoclonal and polyclonal antibodies, ancillary reagents, and customized sourcing for hard-to-find targets—ensuring you have reliable, end-to-end support from concept to clinic.