Phospho-STAT3-S727 Rabbit Polyclonal Antibody for WB, IP, IHC-P, ELISA - P40763

Core Product Specifications and Parameters

Biological Background: STAT3 Function and Localization

• STAT3 (signal transducer and activator of transcription 3), also known as acute-phase response factor (APRF), is a member of the STAT protein family that mediates cellular responses to interleukins, KITLG/SCF, LEP, and other growth factors (PubMed:10688651, PubMed:11294841, PubMed:12359225). Related references: PMID:10688651 PMID:11294841 PMID:12359225

• Upon activation, it recruits coactivators such as NCOA1 or MED1 to the promoter region of target genes (PubMed:15653507, PubMed:16285960, PubMed:17344214). Related references: PMID:15653507 PMID:16285960 PMID:17344214

• May mediate cellular responses to activated FGFR1, FGFR2, FGFR3, and FGFR4 (PubMed:12873986). Related references: PMID:12873986

• Binds to IL6-responsive elements in acute-phase protein gene promoters upon IL6 stimulation (PubMed:12359225), and is activated by IL31 through IL31RA (PubMed:15194700). Related references: PMID:12359225 PMID:15194700

• Regulates inflammatory response by influencing naive CD4+ T-cell differentiation into Th17 or Treg cells; acetylation promotes its transcription activity and cell differentiation, while deacetylation and oxidation of lysine residues by LOXL3 inhibit differentiation (PubMed:28065600, PubMed:28262505). Related references: PMID:28065600 PMID:28262505

• Involved in cell cycle regulation by inducing expression of key genes for G1 to S phase progression, such as CCND1 (PubMed:17344214), and may play an apoptotic role by transactivating BIRC5 under LEP activation (PubMed:18242580). Related references: PMID:17344214 PMID:18242580

• Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity (PubMed:23084476). Related references: PMID:23084476

• Shuttles between cytoplasm and nucleus; plays a crucial role in basal beta cell functions (By similarity), and is involved in oncostatin-M-mediated signaling through type I and II OSM receptor complexes (PubMed:9188471). Related references: PMID:9188471

• Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas; also detected in naive CD4+ T cells and T-helper Th17, Th1, and Th2 subsets (PubMed:31899195). Related references: PMID:31899195

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide matching residues around Ser727 of human STAT3; the antibody is expected to specifically recognize STAT3 only when phosphorylated at this site, based on sequence homology, cross-reactivity with mouse and rat is predicted.
• For Western blot, a band around 88 kDa should be observed; optimization of blocking and antibody dilution may be required for phospho-specific detection.
• For IHC-P, antigen retrieval protocols and fixation conditions may influence phospho-epitope preservation; validation with appropriate positive and negative controls is advised.
• For IP and ELISA applications, verify performance in the relevant sample system, as phospho-antibody binding can be affected by lysis buffer composition and phospho-site accessibility.
• Given the involvement of STAT3 in diverse signaling cascades, consider treating cells with phosphatase inhibitors and appropriate cytokine stimulations to enhance detection of S727 phosphorylation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop platform for IVD raw materials, connecting diagnostic manufacturers with validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing solutions. This phospho-STAT3-S727 antibody supports research in inflammatory signaling, cancer biology, and metabolic regulation. Contact us to explore our full range of phospho-specific antibodies, recombinant proteins, and custom reagent services to accelerate your assay development pipeline.