Phospho-STAT2-Y690 Rabbit Polyclonal Antibody for WB and ELISA - P52630

Core Product Specifications and Parameters

Biological Background: STAT2 Function and Localization

• STAT2 (signal transducer and activator of transcription 2) is a member of the STAT protein family, characterized by an SH2 domain that facilitates phosphotyrosine-dependent dimerization.
• Upon type I interferon (IFN-alpha/beta) stimulation, STAT2 undergoes tyrosine phosphorylation (critical residues include Y690), leading to heterodimerization with STAT1 and association with IRF9 to form the ISGF3 complex. This complex translocates to the nucleus and binds to IFN-stimulated response elements (ISRE), driving transcription of antiviral genes. Related references: PMID:23391734 PMID:9020188
• STAT2 exerts a negative feedback regulatory role in type I IFN signaling by recruiting USP18 to the IFNAR2 subunit of the type I IFN receptor, attenuating the response. Related references: PMID:28165510
• Additionally, STAT2 modulates mitochondrial fission by regulating the phosphorylation status of dynamin-related protein 1 (DNM1L) at Ser-616 and Ser-637, influencing its GTPase activity. Related references: PMID:23391734 PMID:26122121 PMID:9020188
• The protein localizes to both the cytoplasm and nucleus, shuttling in response to activation signals.
• Alternative splicing of STAT2 produces multiple isoforms, and post-translational modifications include phosphorylation, ubiquitination, and acetylation (as indicated by keywords like 'Phosphoprotein', 'Ubl conjugation').
• STAT2 is also implicated in host-virus interactions, serving as a critical mediator of the innate antiviral defense (keyword 'Host-virus interaction', 'Antiviral defense').

Experimental Guidance and Technical Tips

• This antibody was raised against a synthetic phosphorylated peptide corresponding to residues surrounding Y690 of human STAT2. For validation, consider performing peptide competition assays or phosphatase treatment to confirm phospho-specificity.
• In Western blot (WB), use appropriate blocking buffers (e.g., BSA or non-fat milk without phosphates) and consider including phosphatase inhibitors in lysis buffers to preserve phosphorylation states.
• For ELISA, coat the plate with the phospho-peptide antigen and detect with this antibody; include a non-phosphorylated peptide control to assess specificity.
• Cross-reactivity with human, mouse, and rat has been reported; validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutes by providing a comprehensive portfolio of IVD raw materials, including validated phospho-specific antibodies like this anti-STAT2-Y690 antibody. From monoclonal and polyclonal antibodies to ancillary reagents and rare target sourcing, CamelBio simplifies procurement for projects in interferon signaling, antiviral research, and mitochondrial dynamics. Contact us to learn how we can accelerate your assay development with reliable, bulk-ready materials.