Phospho-STAT1-Y701 Rabbit Monoclonal Antibody for WB and ELISA - P42224

Core Product Specifications and Parameters

Biological Background: STAT1 Function and Localization

• Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF, and other cytokines and growth factors. Plays a central role in JAK-STAT signaling cascades. Related references: PMID:12764129, PMID:12855578, PMID:15322115
• Upon type I IFN (IFN-alpha/beta) stimulation, STAT1 becomes tyrosine-phosphorylated at Y701 by JAK kinases, dimerizes with STAT2 and IRF9 to form the ISGF3 complex, which translocates to the nucleus and binds ISRE elements to induce interferon-stimulated genes (ISGs) and establish an antiviral state. Related references: PMID:28753426, PMID:35568036
• In response to type II IFN (IFN-gamma), STAT1 forms a homodimer (GAF) that binds GAS elements, driving transcription of target genes. Tyrosine and serine phosphorylation events are critical for this activation. Related references: PMID:8156998, PMID:26479788
• Activated by KITLG/SCF-KIT signaling, expanding its role beyond interferons. Related references: PMID:15526160
• Participates in FGFR-mediated signaling, potentially through direct interaction or downstream effects. Related references: PMID:19088846
• Following TLR4 endocytosis, STAT1 is phosphorylated at Thr-749 by IKBKB, promoting transcription of ARID5A and IL12B, linking innate immune signals to inflammatory gene expression. Related references: PMID:32209697
• Shuttles between cytoplasm and nucleus; nuclear import is tightly regulated by phosphorylation status. Contains conserved SH2 domain and DNA-binding domain characteristic of STAT family members.
• Also implicated in intestinal immune tolerance: associates with Gasdermin-D p13 fragment to promote CIITA transcription and Tr1 cell differentiation (by similarity).

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic phosphorylated peptide around Y701 of human STAT1; consider employing non-phosphorylated peptide competition or phosphatase treatment to confirm phosphorylation specificity in your assays.
• For western blot, STAT1 runs at approximately 87 kDa. Use appropriate molecular weight markers and validate bands under reducing conditions.
• In ELISA applications, direct or indirect detection formats can be tested; coating with phosphorylated peptide and detecting with HRP-conjugated secondary antibody is a common approach.
• Since STAT1 activation is stimulus-dependent, treat cells with interferon (e.g., IFN-alpha or IFN-gamma) to induce Y701 phosphorylation prior to lysis for robust detection.
• Cross-reactivity with human, mouse, and rat is documented; for other species, align the target sequence around Y701 to assess potential reactivity.

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CamelBio offers comprehensive one-stop sourcing solutions for IVD raw materials, including this Phospho-STAT1-Y701 rabbit monoclonal antibody. We support diagnostic manufacturers and researchers with validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material procurement for JAK-STAT pathway studies and beyond.