Phospho-Src-Y529 Rabbit pAb for WB, IHC-P, IF/ICC, ELISA - P12931

Core Product Specifications and Parameters

Biological Background: Src Function and Localization

• Proto-oncogene tyrosine-protein kinase Src (c-Src) is a ~60 kDa non-receptor tyrosine kinase, encoded by the SRC gene (Gene ID: 6714). Alternative names include ASV, SRC1, THC6, and p60-Src. Three isoforms arise from alternative splicing, with isoform 1 representing the canonical sequence.
• Src mediates signal transduction from diverse receptor types (immune, integrin, growth factor, GPCR), controlling gene transcription, immune response, cell adhesion, cycle progression, apoptosis, migration, and transformation (PubMed:34234773, PubMed:21411625). Related references: PMID:34234773, PMID:21411625
• Through phosphorylation of substrates like AFAP1, cortactin (CTTN), FAK (PTK2), and paxillin, Src orchestrates cytoskeletal reorganization, focal adhesion dynamics, and cell–cell junction signaling.
• Src localizes to the cell membrane, mitochondrial inner membrane, nucleus, cytoplasm, cytoskeleton, and cell junctions, enabling spatially restricted signaling.
• The antibody specifically recognizes Src phosphorylated at Tyr529, a conserved residue in the C-terminal tail whose phosphorylation by Csk kinase maintains the autoinhibited closed conformation. Dephosphorylation or displacement of the pTyr529–SH2 interaction primes Src for activation.
• Src expression is ubiquitous; however, platelets, neurons, and osteoclasts exhibit 5–200‑fold higher levels than most other tissues. Expression has also been detected in skin (PubMed:22294297). Related references: PMID:22294297
• In osteoclasts, Src is essential for bone resorption, acting through a PTK2B/PYK2 complex and phosphorylating CBL to activate PI3K signaling (PubMed:14585963, PubMed:8755529). It also promotes energy production by activating cytochrome c oxidase (PubMed:12615910). Related references: PMID:14585963, PMID:8755529, PMID:12615910
• Src participates in antiviral innate immunity by enhancing RIG-I‑mediated signaling (PubMed:19419966) and mediates IL6‑driven epithelial regeneration through the YAP1‑NOTCH pathway (PubMed:25731159). Related references: PMID:19419966, PMID:25731159

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide corresponding to the Y529 region; ensure that sample preparation preserves phosphorylation (use phosphatase inhibitors) and include non‑phospho controls to verify specificity.
• For Western blot, the predicted molecular weight of Src is ~60 kDa; a band at this size should be observed. Consider using 5% BSA blocking to reduce non‑specific binding.
• In IHC‑P and IF/ICC, antigen retrieval methods may affect phospho‑epitope preservation; gentle citrate‑based retrieval and low‑temperature processing are recommended.
• ELISA detection of phospho‑Src‑Y529 may be used in combination with total Src antibodies to assess site‑specific phosphorylation levels in cell lysates.
• Cross‑reactivity with mouse and rat Src is expected based on sequence homology; validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop sourcing platform for IVD raw materials, including validated phospho‑specific antibodies like this anti‑phospho‑Src‑Y529 pAb. For diagnostic developers and researchers studying kinase signaling, CamelBio also offers matched antibody pairs, bulk ancillary reagents, and customized sourcing of rare targets. From concept to clinic, CamelBio supports manufacturers with integrated raw material supply, technical services, and consulting—simplifying procurement for signal transduction research and beyond.