Phospho-Src-Y419 Rabbit Monoclonal Antibody for WB and ELISA - P12931

Core Product Specifications and Parameters

Biological Background: Src Function and Localization

• SRC is a non-receptor tyrosine kinase that relays signals from immune receptors, integrins, and growth factor receptors, impacting gene expression, cell cycle, and apoptosis. Related references: PMID:34234773
• In focal adhesions, SRC phosphorylates key proteins such as FAK/PTK2 and paxillin (PXN) to regulate adhesion dynamics and migration. Related references: PMID:21411625
• Essential for osteoclast activity and bone resorption; it forms a complex with PTK2B/PYK2 and phosphorylates CBL, leading to PI3K recruitment. Related references: PMID:14585963 PMID:8755529
• Autophosphorylation at Tyr-419 within the activation loop is a hallmark of SRC kinase activation and is targeted by this antibody.
• Subcellularly, SRC localizes to the plasma membrane, focal adhesions, cell junctions, cytoplasm, nucleus, and mitochondrial inner membrane.
• Ubiquitously expressed with elevated levels in platelets, neurons, and osteoclasts; also detected in skin. Related references: PMID:22294297
• Contains SH3, SH2, and tyrosine kinase domains; myristoylation at the N-terminus facilitates membrane association.

Experimental Guidance and Technical Tips

• This antibody was raised against a synthetic phosphorylated peptide corresponding to residues surrounding Tyr419 of human Src; it is expected to specifically recognize the phosphorylated form.
• For Western blotting, include phosphatase inhibitors in lysis buffer to preserve phosphorylation; the predicted molecular weight of Src is ~60 kDa.
• Cross-reactivity has been observed with human and mouse samples; validation in your specific experimental system is recommended.
• When using in ELISA, optimize peptide coating concentration and detection conditions for best signal-to-noise ratio.
• Consider pairing with a total Src antibody to normalize phosphorylation levels and assess activation status.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing platform for high-quality IVD raw materials, offering validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. This anti-Phospho-Src (Y419) antibody supports research into kinase signaling pathways relevant to oncology and bone metabolism. From rare target sourcing to technical consultation, CamelBio provides comprehensive support for diagnostic development from concept to clinic.