Phospho-Smad5-S465 Rabbit Monoclonal Antibody for WB, ELISA - Q99717

Core Product Specifications and Parameters

Biological Background: Smad5 Function and Localization

• SMAD5 (Mothers against decapentaplegic homolog 5, also known as JV5-1, MADH5) is a member of the SMAD family of transcriptional regulators.
• Upon BMP ligand stimulation, SMAD5 is phosphorylated at Ser465 by activated type I BMP receptors, enabling complex formation with SMAD4 and nuclear translocation to regulate transcription.
  Related references: PMID:9442019
• The activated SMAD complex binds Smad Binding Elements (SBEs) in target gene promoters to modulate the outcome of the signaling network.
  Related references: PMID:33510867
• SMAD5 plays critical roles in embryonic development, cell differentiation, angiogenesis, and tissue homeostasis.
  Related references: PMID:12064918 PMID:16516194
• Non-phosphorylated SMAD5 has a cytoplasmic and mitochondrial role in energy metabolism, promoting mitochondrial respiration and glycolysis via interaction with hexokinase 1.
  Related references: PMID:28675158
• SMAD5 is ubiquitously expressed and localizes to the cytoplasm, nucleus, and mitochondrion.
• The protein undergoes multiple post-translational modifications, including phosphorylation and acetylation, and contains a zinc-binding domain essential for DNA interaction.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic phosphopeptide corresponding to residues surrounding Ser465 of human Smad5, ensuring specific detection of the phosphorylated form.
• For Western blotting, use standard protocols with PVDF or nitrocellulose membranes; consider including phosphatase inhibitors in lysis buffers to preserve phosphorylation status.
• In ELISA, the antibody may be used as a capture or detection reagent; optimize coating and detection conditions for the specific assay format.
• Cross-reactivity with human, mouse, and rat Smad5 has been reported; validate in the relevant species and sample type before experimental use.
• The predicted molecular weight of Smad5 is approximately 52 kDa; a band at this size confirms specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including this high-quality phosphorylated Smad5 antibody, to support diagnostic developers and researchers studying BMP signaling and related pathways. As your one-stop sourcing partner, we provide validated antibody pairs, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your project from concept to clinic. Contact us to explore custom procurement solutions tailored to your needs.