Phospho-Smad2-T220 Rabbit pAb - Q15796

Core Product Specifications and Parameters

Biological Background: SMAD2 Function and Localization

• SMAD2 (Mothers against decapentaplegic homolog 2), also known as JV18-1, MADH2, or hMAD-2, is a receptor-regulated SMAD (R-SMAD) protein that functions as an intracellular signal transducer for TGF-beta and activin pathways.
• Upon ligand binding, SMAD2 is phosphorylated by type 1 receptor kinases, forms a complex with SMAD4, and translocates to the nucleus where it binds the TRE element to regulate transcription of target genes.
• SMAD2 positively regulates PDPK1 kinase activity by promoting its dissociation from the inhibitory 14-3-3 protein YWHAQ.
• SMAD2 may act as a tumor suppressor in colorectal carcinoma, as indicated by studies showing its involvement in growth inhibition. Related references: PMID:8752209
• SMAD2 shuttles between the cytoplasm and nucleus in response to signaling activation.
• Highest expression levels are observed in skeletal muscle, endothelial cells, heart, and placenta.
• SMAD2 undergoes multiple post-translational modifications including phosphorylation, acetylation, and ubiquitination, which fine-tune its activity and stability.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around Thr220; therefore, this antibody specifically detects SMAD2 phosphorylated at this residue.
• For Western blotting, a band around 52 kDa is expected. Use phosphatase inhibitors during cell lysis to preserve phosphorylation.
• Consider using TGF-beta stimulation to induce SMAD2 phosphorylation as a positive control in your experimental system.
• For IHC-P, optimize antigen retrieval conditions and include appropriate controls for phospho-epitope stability.
• Cross-reactivity with human, mouse, and rat samples is reported; validate in your specific tissue or cell line of interest.

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CamelBio is your one-stop sourcing partner for IVD raw materials, providing a comprehensive portfolio of validated antibodies, including phospho-specific monoclonal and polyclonal antibodies like Phospho-Smad2-T220. We support diagnostic developers and researchers with bulk reagent supply, customized sourcing of rare-target antibodies, and ancillary reagents for TGF-beta signaling studies. Streamline your assay development from concept to clinic with CamelBio's integrated procurement solutions.