Phospho-Smad2-S465/S467 + Smad3-S423/S425 Rabbit mAb for WB, ELISA - Q15796/P84022

Core Product Specifications and Parameters

Biological Background: SMAD2 Function and Localization

• SMAD2 (Mothers against decapentaplegic homolog 2), also known as MADH2 or MADR2, is a receptor-regulated SMAD (R-SMAD) that serves as an intracellular signal transducer for TGF-beta and activin type 1 receptor kinases.
• Upon ligand binding, SMAD2 is phosphorylated at C-terminal serine residues S465/S467, leading to complex formation with SMAD4 and nuclear translocation, where it binds TRE elements to regulate gene transcription.
• It positively modulates PDPK1 kinase activity by promoting dissociation from the 14-3-3 protein YWHAQ, a key negative regulator.
• SMAD2 may function as a tumor suppressor in colorectal carcinoma. Related references: PMID:8752209
• Subcellularly, SMAD2 localizes to both the cytoplasm and nucleus, with dynamic shuttling controlled by phosphorylation status.
• Tissue expression is prominent in skeletal muscle, endothelial cells, heart, and placenta.
• Post-translational modifications including phosphorylation, acetylation, and ubiquitination fine-tune SMAD2 activity and stability.
• This antibody detects dual phosphorylation at S465/S467 of SMAD2 and the homologous sites S423/S425 of SMAD3, both critical for activation in TGF-beta signal transduction.

Experimental Guidance and Technical Tips

• The rabbit monoclonal antibody was raised against a synthetic phosphopeptide surrounding S465/S467 of human SMAD2, ensuring specificity for the activated phosphorylated state.
• For Western blotting, include phosphatase inhibitors in lysis buffers to preserve target phosphorylation. Expected major bands: ~52 kDa (SMAD2) and ~48 kDa (SMAD3); additional bands at ~25, 35, and 43 kDa may represent cleavage products or isoforms. Verify specificity with phosphatase-treated controls or siRNA knockdown.
• For ELISA, consider normalizing phospho-signal to total SMAD2/3 levels using a pan antibody; optimize experimental conditions empirically.
• Cross-reactivity has been observed with human, mouse, and rat proteins; confirm endogenous phosphorylation levels in your experimental system with appropriate TGF-beta stimulation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes a streamlined path to IVD raw material procurement. This phospho-SMAD2/3 antibody is part of our comprehensive portfolio supporting TGF-beta signaling and oncology research. From validated antibody pairs and optimized monoclonal/polyclonal reagents to bulk ancillary materials and rare target sourcing, CamelBio bridges the gap from concept to clinic.