Phospho-Smad1-S206 Rabbit pAb for WB, ELISA - Q15797

Core Product Specifications and Parameters

Biological Background: Smad1 Function and Localization

• Smad1 (Mothers against decapentaplegic homolog 1, MADH1, BSP1) is a key intracellular mediator of the bone morphogenetic protein (BMP) signaling pathway, belonging to the SMAD family of transcription factors. It is also known as JV4-1, MADR1, and BSP-1.
• Functions as a transcriptional modulator regulating embryonic development, cell differentiation, and tissue homeostasis by binding to Smad Binding Elements (SBEs) in target gene promoters (PubMed:9335504, PubMed:33667543). Related references: PMID:9335504 PMID:33667543
• Upon BMP receptor activation, Smad1 is phosphorylated at the C-terminal SSXS motif (including Ser206) by type I BMP receptors, enabling complex formation with SMAD4 and nuclear translocation to regulate transcription (PubMed:33667543). Related references: PMID:33667543
• Subcellular localization is both cytoplasmic and nuclear; in the absence of signaling, Smad1 resides mainly in the cytoplasm, and upon activation, it accumulates in the nucleus.
• Ubiquitously expressed with highest levels in heart and skeletal muscle, reflecting its broad roles in tissue patterning and homeostasis.
• Post-translational modifications include phosphorylation, acetylation, and ubiquitination, which modulate its stability, activity, and interactions.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide around Ser206; consider using phosphatase inhibitors during sample preparation to maintain the phosphorylation state.
• For Western blotting (WB), a band of approximately 52 kDa is expected. Validate the antibody with appropriate positive and negative controls (e.g., untreated vs. BMP-treated lysates).
• Cross-reactivity is reported for human, mouse, and rat; confirm specificity in your experimental model by siRNA knockdown or peptide competition assays.
• ELISA applications may require titration to determine the optimal coating concentration and detection range.

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CamelBio serves as a comprehensive sourcing partner for IVD manufacturers, providing one-stop access to essential raw materials like this phospho-Smad1 (Ser206) antibody for BMP signaling research. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. We help streamline your diagnostic development from concept to clinic with reliable, traceable raw-material supply.