Phospho-SIRT1-S47 Rabbit mAb for Western Blot and ELISA - Q96EB6

Core Product Specifications and Parameters

Biological Background: SIRT1 Function and Localization

• NAD-dependent protein deacetylase that links transcriptional regulation to intracellular energetics and coordinates cell cycle, DNA damage response, metabolism, apoptosis, and autophagy.
  Related references: PMID:11672523, PMID:12006491, PMID:14976264
• Modulates chromatin structure by deacetylating histones H3, H4, H2A, and H1, and promotes histone methylation and DNA methylation changes, leading to transcriptional repression.
  Related references: PMID:15469825
• Deacetylates a wide range of transcription factors and coregulators, including p53, FOXO3, RELA/p65, MYC, and HIF1A, thereby regulating their stability, transactivation, and subcellular localization.
  Related references: PMID:14980222, PMID:15152190, PMID:14976264, PMID:15126506
• Serves as a sensor of the NAD+/NADH ratio and is activated by caloric restriction and low glucose; participates in metabolic regulation in liver, muscle, and adipose tissue.
  Related references: PMID:15205477
• Localizes to the nucleus, PML bodies, cytoplasm, and mitochondria, reflecting its diverse functions in transcriptional regulation, DNA repair, and apoptosis.
• Widely expressed, with critical roles in skeletal muscle differentiation, adipogenesis, circadian rhythm control, and the maintenance of genomic integrity.
  Related references: PMID:19188449, PMID:18662546, PMID:18004385
• Alternative splicing generates isoforms including isoform 2 and a catalytically inactive 75 kDa form (75SirT1), which exhibit distinct activities and expression patterns.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide surrounding Ser47 of human SIRT1, ensuring specificity for the phosphorylated epitope. For Western blot, use lysis buffers with phosphatase inhibitors to preserve phosphorylation and consider treating samples with alkaline phosphatase as a negative control to confirm phospho-specificity.
• For ELISA, include both phosphorylated and non-phosphorylated peptide competitors to verify antibody specificity.
• This rabbit monoclonal antibody provides consistent batch-to-batch reproducibility and may be compatible with other applications such as immunoprecipitation or immunofluorescence following appropriate validation in the relevant sample system.
• Recommended to follow standard loading control practices (e.g., beta-actin or GAPDH) and to use appropriate molecular weight markers.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to a broad portfolio of IVD raw materials, including validated phosphorylation-specific antibodies like this anti-SIRT1 (pSer47) clone. Our integrated supply chain supports your development from early-stage target discovery through clinical validation, offering bulk ancillary reagents, custom antibody services, and rare-target sourcing. Leverage our expertise to streamline your next-generation metabolic or oncology assay development with reliable, high-quality antibody reagents.