Phospho-SHP2-Y542 Rabbit pAb - Q06124

Core Product Specifications and Parameters

Biological Background: SHP2 Function and Localization

• SHP2 (PTPN11) functions downstream of various receptor and cytoplasmic protein tyrosine kinases, relaying signals from the cell surface to the nucleus and positively regulating the MAPK pathway.
  Related references: PMID:10655584, PMID:14739280, PMID:18559669
• It dephosphorylates multiple substrates, including GAB1, ARHGAP35, EGFR, ROCK2, CDC73, and SOX9, thereby modulating diverse cellular processes.
  Related references: PMID:28074573, PMID:18559669, PMID:26742426
• In T cells, SHP2 is recruited by phosphorylated PDCD1 to inhibit T-cell receptor (TCR) signaling by dephosphorylating key proximal signaling molecules.
  Related references: PMID:32184441
• The protein localizes to both the cytoplasm and nucleus, consistent with its roles in signal transduction and transcriptional regulation.
• Widely expressed, with highest levels observed in heart, brain, and skeletal muscle.
• Contains two N-terminal SH2 domains and a C-terminal tyrosine phosphatase domain; post-translational modifications include acetylation and phosphorylation, which regulate its activity and interactions.
• PTPN11 is annotated with keywords such as disease variant and deafness, indicating its involvement in human pathologies.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic phosphopeptide encompassing phosphorylated Y542 of human SHP2; it is designed to specifically recognize the Y542-phosphorylated form.
• For Western blotting, include phosphatase inhibitors in lysis buffers and consider running dephosphorylated controls (e.g., phosphatase-treated lysates) to confirm phospho-specificity.
• Validated applications include WB and ELISA using human, mouse, and rat samples. Cross-reactivity with mouse and rat is expected due to high sequence homology around the Y542 site.
• In ELISA applications, coating with the phosphorylated peptide or using a competitive peptide inhibition assay can help verify specificity.
• Use a compatible anti-rabbit secondary antibody and optimize primary antibody dilutions empirically for your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw materials, offering a seamless bridge from research to diagnostic manufacturing. This Phospho-SHP2-Y542 Rabbit pAb exemplifies our commitment to providing high-quality phospho-specific tools for signaling research. Beyond individual antibodies, CamelBio delivers one-stop sourcing solutions—including validated antibody pairs, bulk-optimized monoclonal and polyclonal antibodies, and custom procurement for rare targets—to accelerate your assay development and production timelines.