Phospho-S6 Ribosomal Protein (RPS6)-S240/244 Rabbit mAb for WB, IHC-P, ELISA - P62753

Core Product Specifications and Parameters

Biological Background: RPS6 Function and Localization

• RPS6 (40S ribosomal protein S6, also known as eS6 or phosphoprotein NP33) is a core component of the 40S small ribosomal subunit, essential for ribosome assembly and protein synthesis (PubMed:23636399, PubMed:8706699). Related references: PMID:23636399 PMID:8706699
• It plays a critical role in cell growth and proliferation by selectively regulating the translation of specific mRNA classes (PubMed:17220279). Related references: PMID:17220279
• RPS6 is part of the small subunit (SSU) processome, the earliest precursor of the small eukaryotic ribosomal subunit, participating in pre-rRNA processing, RNA folding, and quality control (PubMed:34516797). Related references: PMID:34516797
• The protein localizes predominantly in the cytoplasm, but is also detected in the nucleus and nucleolus, reflecting its dual role in ribosome biogenesis and translation.
• RPS6 undergoes extensive post-translational modifications, including phosphorylation at Ser240 and Ser244 (critical for translation regulation and mTORC1 pathway activity), acetylation, ADP-ribosylation, hydroxylation, and ubiquitination.
• The unmodified molecular weight is approximately 28.7 kDa, while the phosphorylated form typically migrates at ~29 kDa on SDS-PAGE.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide spanning residues around S240 and S244 of human RPS6; this antibody is expected to specifically recognize the phosphorylated form of the protein.
• For Western blotting, anticipate a band at around 29 kDa; confirm specificity using phosphatase-treated lysates or mTOR pathway inhibitors as controls.
• In immunohistochemistry (IHC-P), optimize antigen retrieval and antibody dilution based on tissue type and fixation conditions, as ribosomal proteins are abundant and may require careful titration.
• The reported cross-reactivity with mouse and rat suggests suitability for mammalian model systems; include appropriate positive and negative controls to validate species reactivity.
• ELISA applications may require pairing with a suitable capture or detection antibody; consider testing with recombinant phospho-RPS6 or cell lysates.

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CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated monoclonal antibodies such as this anti-phospho-RPS6 (S240/244) antibody to support diagnostic development and mTOR pathway research. Our platform streamlines procurement of optimized phospho-specific reagents, bulk ancillary materials, and rare target antibodies, enabling a seamless transition from concept to clinical application.