Phospho-S6 Ribosomal Protein (RPS6)-S235/236 Rabbit mAb - P62753

Core Product Specifications and Parameters

Biological Background: RPS6 Function and Localization

• RPS6 encodes the small ribosomal subunit protein eS6 (also known as 40S ribosomal protein S6 or phosphoprotein NP33), an integral component of the eukaryotic translation machinery.
• As a structural part of the 40S small ribosomal subunit, RPS6 is essential for protein synthesis and contributes to the selective translation of specific mRNA subsets, thereby controlling cell growth and proliferation. Related references: PMID:23636399, PMID:8706699, PMID:17220279
• RPS6 participates in the assembly of the small subunit (SSU) processome within the nucleolus, aiding in pre-rRNA processing steps such as folding, modification, cleavage, and targeted degradation of pre-ribosomal RNA. Related references: PMID:34516797
• The protein localizes to the cytoplasm, nucleus, and nucleolus, consistent with its dual functions in ribosome biogenesis and mature ribosome activity.
• RPS6 is a phosphoprotein subject to multiple post-translational modifications, including phosphorylation at serine residues S235 and S236, acetylation, ADP-ribosylation, hydroxylation, and ubiquitination, which modulate its function.
• RPS6 phosphorylation at S235 and S236 is rapidly induced upon growth factor stimulation, serving as a key event in translation activation and often assessed in cell biology assays.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide corresponding to the dual-phosphorylated S235/S236 region of human RPS6; antibody reactivity is likely directed against this phosphorylation motif.
• For Western blotting, include phosphatase inhibitors in lysis buffers to preserve phosphorylation status; expect a band near 29 kDa.
• For IHC-P, antigen retrieval and phosphatase inhibitor pretreatment may improve detection of the phospho-epitope.
• The antibody is listed for WB, IHC-P, and ELISA; validate in your model system using appropriate controls (e.g., peptide competition or phosphatase treatment).
• Cross-reactivity with mouse and rat is reported; verification in the target species is recommended.

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CamelBio offers diagnostic developers a one-stop procurement channel for high-quality IVD raw materials, such as this phospho-RPS6 (S235/236) rabbit monoclonal antibody, essential for studying ribosomal protein phosphorylation and related signaling pathways. Backed by a global network, we supply validated antibody pairs, optimized monoclonals and polyclonals, bulk reagents, and rare-target raw materials, ensuring a seamless path from research to clinical application.