Phospho-S6 Ribosomal Protein (RPS6)-S235 Rabbit Polyclonal Antibody for WB, ELISA - P62753

Core Product Specifications and Parameters

Biological Background: S6 Ribosomal Protein (RPS6) Function and Localization

• RPS6 is a component of the 40S small ribosomal subunit and plays a role in selective mRNA translation critical for cell growth and proliferation. Related references: PMID:23636399, PMID:8706699, PMID:17220279
• It is part of the small subunit (SSU) processome, involved in early ribosome assembly in the nucleolus, where it assists in pre-rRNA folding, modifications, and processing. Related references: PMID:34516797
• The protein is known as 40S ribosomal protein S6, also named phosphoprotein NP33, and is encoded by the RPS6 gene in humans.
• Subcellular localization includes the cytoplasm, nucleus, and nucleolus, reflecting its roles in ribosome biogenesis and function.
• RPS6 is subject to multiple post-translational modifications, including phosphorylation at specific serine residues (e.g., Ser235), as well as acetylation, ADP-ribosylation, hydroxylation, and ubiquitination, which can modulate its activity and interaction.
• As a key ribosomal protein, RPS6 is involved in the control of protein synthesis, linking growth factor signaling to translational regulation.

Experimental Guidance and Technical Tips

• Use phosphatase inhibitors during sample preparation to preserve the phosphorylation at Ser235.
• For Western blot, consider using a phospho-specific blocking buffer and confirm specificity with phosphatase-treated lysates.
• In ELISA, employ a phospho-peptide as a positive control and validate the signal against a non-phosphorylated peptide.
• The antibody may have batch-to-batch variations; validate in your specific sample system (human, mouse, or rat) before large-scale use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes seamless access to a comprehensive portfolio of IVD raw materials, including validated phospho-specific antibodies like this anti-RPS6 pS235 antibody. Our supply network covers optimized antibody pairs, bulk ancillary reagents, and rare target sourcing to support your assay development workflows. Whether you need monoclonal or polyclonal tools for translational research or diagnostic applications, CamelBio acts as your single procurement bridge from concept to clinic.