Phospho-RPS6KA5-T581 Rabbit pAb - O75582

Core Product Specifications and Parameters

Biological Background: RPS6KA5 Function and Localization

• Ribosomal protein S6 kinase alpha-5 (RPS6KA5), also known as MSK1, is a serine/threonine-protein kinase belonging to the RSK family, existing in multiple isoforms due to alternative splicing.
• It is required for the mitogen- or stress-induced phosphorylation of transcription factors CREB1 and ATF1, thereby regulating immediate-early gene expression in response to stimuli such as EGF and UV irradiation.
  Related references: PMID:11909979, PMID:9873047
• RPS6KA5 plays a critical role in inflammatory signaling by controlling RELA (NF-kB p65) transcriptional activity, including mediating glucocorticoid-induced repression of inflammatory genes.
  Related references: PMID:12628924, PMID:18511904
• It phosphorylates STAT3 at Ser-727 in response to erythropoietin, modulating STAT3 transcriptional potential.
  Related references: PMID:12763138
• The kinase directly phosphorylates histone H3 (at Ser-10 and possibly Ser-28) and histone H2A (at Ser-1), thereby contributing to chromatin remodeling and gene activation or repression.
  Related references: PMID:15010469, PMID:12773393
• RPS6KA5 also phosphorylates ETV1/ER81 at Ser-191 and Ser-216, regulating its transcriptional activity with implications for development and tumorigenesis.
  Related references: PMID:12569367
• It targets other substrates such as HMGN1/HMG14 and TRIM7, and has been implicated in neuronal cell death and macrophage anti-inflammatory responses.
  Related references: PMID:12773393, PMID:25851810
• Subcellularly, RPS6KA5 localizes to both the nucleus and cytoplasm, consistent with its roles in signal transduction and gene regulation.
• It is widely expressed, with highest levels in heart, brain, and placenta; lower levels in lung, kidney, and liver.

Experimental Guidance and Technical Tips

• Since the immunogen is a synthetic phosphorylated peptide around Thr581, verify phospho-specificity by comparing reactivity against phosphorylated and non-phosphorylated peptide or phosphatase-treated samples.
• For Western blotting, expect a band around 90 kDa; consider using whole-cell lysates from stimulated human or rat cells (e.g., EGF-treated) to enhance detection of phosphorylated RPS6KA5.
• In ELISA, the antibody can be used to detect and quantify phospho-RPS6KA5-T581 levels; appropriate blocking and washing conditions should be optimized.
• Cross-reactivity with rat has been reported; validate antibody performance in the relevant species and sample type before experimental use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop sourcing solution for IVD raw materials, supporting diagnostic developers from concept to clinic. For researchers and manufacturers studying kinase signaling and stress response pathways, we supply validated phospho-specific antibodies like this anti-Phospho-RPS6KA5-T581 pAb, along with monoclonal/polyclonal antibodies, bulk reagents, and rare target sourcing. Contact us to streamline your procurement of high-quality critical raw materials.