Phospho-Rpb1 CTD-T4 Rabbit mAb for WB, ELISA - P24928

Core Product Specifications and Parameters

Biological Background: DNA-directed RNA polymerase II subunit RPB1 Function and Localization

• DNA-directed RNA polymerase II subunit RPB1 is the catalytic core of Pol II, responsible for synthesizing mRNA precursors and non-coding RNAs in a transcription cycle encompassing initiation, elongation, and termination. Related references: PMID:23748380, PMID:27193682, PMID:30190596, PMID:28108474
• RPB1 forms the active center with subunit RPB2, catalyzing nucleotide addition via a Mg²⁺-coordinated DxDGD motif and proofreads misincorporated nucleotides through 3′→5′ exonuclease activity. Related references: PMID:8381534, PMID:9852112, PMID:30190596
• The unique C-terminal domain (CTD) of RPB1, composed of 52 heptapeptide repeats, serves as a dynamic platform for transcription-coupled processes; phosphorylation at Ser5 promotes promoter escape, while Ser2 phosphorylation is critical for elongation and pre-mRNA processing. Acetylation and methylation of CTD lysines further fine-tune gene expression. Related references: PMID:24207025, PMID:26124092
• Beyond DNA-dependent transcription, RPB1 possesses RNA-dependent RNA polymerase activity that extends non-coding RNAs and can act as replicase/transcriptase for Hepatitis delta virus.
• RPB1 localizes primarily in the nucleus and on chromosomes, but can also be found in the cytoplasm.
• Post-translational modifications of RPB1 include phosphorylation, acetylation, methylation, ubiquitination, and ISGylation, regulating its activity and interactions.
• Mutations in POLR2A are associated with neurodevelopmental disorders including intellectual disability (NEDHIB).

Experimental Guidance and Technical Tips

• When performing Western blotting, include phosphatase inhibitors in lysis buffers to preserve the T4 phosphorylation, and use a gradient or high‑percentage resolving gel to clearly separate the 217 kDa band.
• For ELISA, consider coating with a phosphorylated peptide or phospho‑enriched protein extracts to confirm specificity for the modified epitope.
• The antibody’s cross‑reactivity with human, mouse, and rat suggests a conserved T4 motif; validate in the relevant species and sample type for your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials for transcription and chromatin research, including validated antibody pairs, optimized monoclonal and polyclonal antibodies targeting RNA polymerase II modifications, and bulk ancillary reagents. For specialized needs such as rare phospho‑specific targets or custom immunogen design, our sourcing services can help you secure the right materials efficiently.