Phospho-Rpb1 CTD-S2/S5 Rabbit mAb - P24928

Core Product Specifications and Parameters

Biological Background: DNA-directed RNA polymerase II subunit RPB1 Function and Localization

• DNA-directed RNA polymerase II subunit RPB1 (also known as POLR2A) is the largest and catalytic core component of RNA polymerase II, responsible for synthesizing mRNA precursors and many functional non-coding RNAs. Related references: PMID:23748380 PMID:27193682 PMID:30190596
• The Pol II transcription cycle includes initiation, elongation, and termination. During initiation, the pre-initiation complex assembles at promoters; elongation involves processive RNA synthesis; termination releases the transcript. Related references: PMID:23748380 PMID:27193682 PMID:28108474
• The enzyme catalyzes nucleotide addition via a two-metal-ion mechanism, with POLR2A contributing a Mg²⁺-coordinating DxDGD motif and POLR2B providing additional coordination. Related references: PMID:30190596 PMID:8381534 PMID:9852112
• Possesses 3'→5' exonuclease proofreading activity, enabling removal of misincorporated nucleotides. Related references: PMID:8381534
• The unique C-terminal domain (CTD) consists of 52 heptapeptide repeats (YSPTSPS) and undergoes dynamic phosphorylation, primarily at Ser2 and Ser5, which regulates promoter escape, elongation, and mRNA processing. Related references: PMID:24207025 PMID:26124092
• CTD lysine acetylation and methylation balance gene expression, with acetylation promoting transcription of growth-factor-induced genes and methylation generally repressing expression. Related references: PMID:24207025 PMID:26124092
• Additionally, RPB1 can function as an RNA-dependent RNA polymerase, extending non-coding RNAs, and acts in the replication of hepatitis delta virus.
• Subcellular localization: predominantly nuclear, but also found in the cytoplasm and associated with chromosomes.

Experimental Guidance and Technical Tips

• When performing Western blot, ensure lysis buffers contain phosphatase inhibitors to preserve CTD phosphorylation. Include a phosphatase-treated control to confirm phospho-specificity.
• For chromatin immunoprecipitation (ChIP) and CUT&Tag, this antibody may help profile RNA polymerase II occupancy and Ser2/Ser5 phosphorylation patterns across the genome.
• In ELISA, the antibody can be used to quantify phospho-POLR2A levels in lysates or purified samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio accelerates diagnostic development by providing a comprehensive range of IVD raw materials and technical support—from concept to clinic. For transcription research targeting RNA polymerase II and CTD modifications, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. Partner with us to secure reliable, high-quality materials for your assays.