Phospho-RB-S795 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P06400

Core Product Specifications and Parameters

Biological Background: Retinoblastoma-associated protein Function and Localization

• Retinoblastoma-associated protein (RB1, also known as pRb, p105-Rb, p110-RB1, pp110) is a tumor suppressor protein encoded by the RB1 gene in humans.
• RB1 is a key regulator of the G1/S transition of the cell cycle. In its hypophosphorylated state, it binds and inhibits E2F transcription factors, repressing transcription of genes required for S-phase entry. Related references: PMID:10499802
• Cyclin-dependent kinase (CDK)-mediated phosphorylation of RB1 disrupts its interaction with E2Fs, releasing the transcriptional repression and allowing cell cycle progression. Related references: PMID:10499802
• RB1 also promotes the G0-G1 transition through phosphorylation by CDK3/cyclin-C, further highlighting its regulatory role in cell cycle entry and exit. Related references: PMID:15084261
• Subcellularly, RB1 is predominantly nuclear but can also be found in the cytoplasm, consistent with its functions in transcription regulation and chromatin organization.
• RB1 is involved in heterochromatin formation and epigenetic silencing by recruiting histone methyltransferases (e.g., SUV39H1, KMT5B, KMT5C) and histone deacetylases, thereby maintaining chromatin structure.
• Tissue expression includes the retina and foreskin keratinocytes, where RB1 protein has been detected.
• Additionally, RB1 is targeted by viral oncoproteins such as SV40 large T antigen, HPV E7, and adenovirus E1A, which disrupt RB1-E2F complexes to promote viral replication.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide corresponding to residues surrounding Ser795 of human RB. When assessing specificity, consider comparing signals with those from a non-phospho-specific RB antibody or phosphatase-treated samples.
• For Western blotting, RB1 is a large protein (approximately 106 kDa); ensure proper transfer conditions and use prestained markers to confirm molecular weight.
• In immunohistochemistry (IHC-P), antigen retrieval and blocking steps may influence staining; optimize these parameters in your specific tissue system.
• The antibody shows cross-reactivity with mouse and rat RB1, enabling its use in studies involving rodent cell lines or tissues; still, validate expression levels and phosphorylation state in your model.
• Given its application in ELISA, ensure that the phosphorylated epitope is preserved in the coating buffer and that blocking agents do not interfere with phospho-specific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutes comprehensive access to IVD raw materials, including this high-quality phospho-RB1 antibody. Complementing our catalog are validated antibody pairs, bulk ancillary reagents, and custom sourcing solutions for rare targets, supporting your cell cycle and oncology assay development from concept to clinic. Let CamelBio streamline your supply chain with reliable, one-stop procurement.