Phospho-Raptor-Ser792 Rabbit Monoclonal Antibody for WB, ELISA - Q8N122

Core Product Specifications and Parameters

Biological Background: Regulatory-associated protein of mTOR Function and Localization

• RPTOR is a key component of the mechanistic target of rapamycin complex 1 (mTORC1), which acts as a central nutrient sensor stimulating anabolic reactions and cell growth. Related references: PMID:12150925 PMID:12150926 PMID:12747827
• In response to nutrients and growth factors, mTORC1 is recruited to the lysosome membrane, where RPTOR mediates this recruitment via interaction with Rag GTPases. Related references: PMID:12747827 PMID:24403073 PMID:26588989
• RPTOR promotes substrate specificity by binding to TOS motif-containing proteins and directing them to the MTOR kinase domain for phosphorylation, including key downstream effectors RPS6KB1 and EIF4EBP1. Related references: PMID:12747827
• mTORC1 also inhibits catabolic pathways by phosphorylating autophagy initiation components ULK1 and ATG13, as well as transcription factor TFEB. Related references: PMID:12150925 PMID:12150926 PMID:12747827
• The protein localizes to the lysosome membrane, cytoplasm, and cytoplasmic granules, consistent with its dynamic role in nutrient sensing.
• RPTOR is a phosphoprotein (as indicated by its keyword annotation), subject to regulatory post-translational modifications; acetylation and ubiquitination have also been observed.
• Highly expressed in skeletal muscle, with lower expression in brain, lung, small intestine, kidney, and placenta; widely expressed across tissues with highest levels in nasal mucosa and pituitary.
• Gene aliases include KOG1 and Mip1; the protein is also known as p150 target of rapamycin (TOR)-scaffold protein.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to the phosphorylated Ser792 site; for WB, use human cell lines such as HeLa or HEK293 known to express RPTOR.
• Validate phospho-specificity by treating cell lysates with lambda phosphatase to confirm loss of signal, or by using non-phospho peptide competition in ELISA.
• For ELISA, consider coating with the phosphorylated peptide and detecting with the antibody; optimize coating concentration and blocking conditions.
• RPTOR is a large protein (~149 kDa); ensure adequate protein transfer and use appropriate gel percentage (e.g., 6-8% SDS-PAGE) for WB.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop supplier of IVD raw materials, offering validated antibody pairs, recombinant antigens, and bulk ancillary reagents for mTOR signaling research. From concept to clinic, we support diagnostic manufacturers with optimized monoclonal antibodies like this Phospho-Raptor-Ser792 mAb, alongside technical consulting and rare target sourcing. Partner with us to accelerate your assay development in nutrient-sensing and metabolic disease biomarker studies.