Phospho-Rac1/Cdc42-S71 Rabbit mAb for WB, ELISA - P60953 / P63000

Core Product Specifications and Parameters

Biological Background: Phospho-Rac1/Cdc42-S71 Function and Localization

This antibody recognizes phosphorylated serine 71 of both human Rac1 and CDC42. The following background focuses on CDC42 (P60953), a key member of the Rho GTPase family.

• Cell division control protein 42 homolog (CDC42) is a plasma membrane-associated small GTPase that cycles between active GTP-bound and inactive GDP-bound states, regulating diverse cellular processes.
• Active CDC42 binds effector proteins to regulate epithelial cell polarization, bipolar spindle attachment during mitosis (PubMed:15642749), and cell migration (PubMed:17038317; PubMed:22843693). Related references: PMID:15642749 PMID:17038317 PMID:22843693
• In neurons, CDC42 promotes filopodia formation and dendritic spine structural plasticity, relaying CaMKII signals for synapse-specific long-term modulation (PubMed:14978216). Related references: PMID:14978216
• Required for DOCK10-mediated spine formation in Purkinje cells and hippocampal neurons, and facilitates DOCK11-dependent filopodia and podosome formation in podocytes (PubMed:33523862). Related references: PMID:33523862
• Involved in phagocytosis by organizing the F-actin cytoskeleton to form phagocytic cups (PubMed:26465210) and in epithelial cell-cell junction remodeling via PLEKHG4B activation (PubMed:33310911). Related references: PMID:26465210 PMID:33310911
• Negatively regulates SEPTIN12 polymerization, potentially impacting spermatogenesis (PubMed:30189608). Related references: PMID:30189608
• Subcellularly, CDC42 localizes to the cell membrane, cytoplasm, centrosome, spindle, midbody, dendrites, and secretory vesicles such as acrosomes.
• Post-translationally modified by phosphorylation, prenylation, methylation, and ubiquitination; identified as a phosphoprotein in proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around S71 of human Rac1/Cdc42. Verify reactivity in your sample system, especially if working with other Rho GTPase family members due to sequence homology.
• Suitable for Western blotting (WB) and ELISA; in WB, a specific band around 24 kDa may be detected in human cell lysates. Use phosphatase-treated controls or induction/starvation conditions to confirm phospho-specificity.
• For ELISA, coating with phosphorylated versus non-phosphorylated peptides can help assess phospho-specific binding.
• Optimal working dilutions should be determined empirically; a typical starting range for WB is 1:500–1:2000, but batch-specific titration is recommended.
• Store at -20°C; shipped on ice bags. Avoid repeated freeze-thaw cycles to maintain activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic and research teams with reliable one-stop access to IVD raw materials, from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents and rare-target sourcing. For projects targeting phosphorylated Rac1/Cdc42 signaling, we can help you secure the right raw materials and technical insights to move from research concept to clinical application efficiently.