Phospho-Rac1/2/3-S71 Rabbit pAb for WB, ELISA - P63000/P15153/P60763

Core Product Specifications and Parameters

Biological Background: Rac1/2/3 Function and Localization

• Rac1 is a plasma membrane-associated small GTPase that cycles between an active GTP-bound state and an inactive GDP-bound state, thereby regulating diverse cellular processes including secretory trafficking, phagocytosis of apoptotic cells, epithelial cell polarization, and growth factor-induced membrane ruffling.
  Related references: PMID:1643658 | PMID:22843693 | PMID:23512198
• Stimulates PKN2 kinase activity, contributing to downstream signaling cascades.
  Related references: PMID:9121475
• Required for DSG3 translocation to cell–cell junctions and the organization of cortical F‑actin bundles, anchoring the actin cytoskeleton at cell junctions.
  Related references: PMID:22796473
• Acts as a subunit of the phagocyte NADPH oxidase complex, mediating the transfer of electrons from cytosolic NADPH to O2 to generate superoxide anions.
  Related references: PMID:38355798
• In neurons, participates in dendritic spine morphogenesis and synapse formation, and regulates GABA(A) receptor synaptic stability through PAK1-mediated F‑actin stabilization (by similarity).
• Isoform B possesses accelerated GEF-independent GDP/GTP exchange and impaired GTP hydrolysis, a property partially restored by GTPase-activating proteins; it is predominantly expressed in skin and intestinal epithelia and is upregulated in colorectal tumors.
  Related references: PMID:14625275
• Localizes to the cell membrane, melanosomes, cytoplasm, lamellipodia, dendrites, synapses, nucleus, and ruffle membrane, reflecting its multifaceted roles in motility and signaling.
• Classified as a phosphoprotein, GTP-binding protein, and lipoprotein, and is subject to prenylation, palmitoylation, and ubiquitin conjugation.

Experimental Guidance and Technical Tips

• Because this antibody recognizes phosphorylation at Ser71, include phosphatase inhibitors (e.g., sodium orthovanadate, NaF) in lysis buffers to preserve the phospho‑epitope.
• Verify phosphorylation‑dependent detection by treating samples with lambda phosphatase or by performing peptide competition with the non‑phosphorylated counterpart.
• For Western blotting, a band around 21 kDa is expected; optimize blocking conditions (e.g., 5% BSA in TBST) to minimize nonspecific binding.
• In ELISA, immobilize the phospho‑peptide or coat cells/extracts directly and use a non‑phospho control to assess specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a trusted one‑stop sourcing partner for IVD raw materials, offering manufacturers, laboratories, and research institutes streamlined access to validated antibodies, bulk ancillary reagents, and consulting support—from concept to clinical application. For researchers studying small GTPase signaling, cytoskeletal dynamics, or NADPH oxidase biology, this anti‑phospho‑Rac1/2/3 antibody is part of a broad portfolio that also includes matched antibody pairs and customized monoclonal/polyclonal antibodies for rare or emerging targets.