Phospho-PSD93/chapsyn-110/DLG2-Y340 Rabbit pAb for WB and ELISA - Q15700

Core Product Specifications and Parameters

Biological Background: Disks Large Homolog 2 (DLG2) Function and Localization

• Disks large homolog 2 (DLG2), also known as postsynaptic density protein PSD-93 or chapsyn-110, is a scaffold protein encoded by the DLG2 gene.
• It is required for the perception of chronic pain through NMDA receptor signaling. DLG2 regulates the surface expression of NMDA receptors in dorsal horn neurons of the spinal cord (by similarity).
• DLG2 interacts with the cytoplasmic tails of NMDA receptor subunits and inward rectifying potassium channels, contributing to synaptic organization.
• As a component of the postsynaptic density at excitatory synapses, it plays a role in maintaining synaptic stability, particularly at cholinergic synapses.
• Subcellularly, DLG2 localizes to the cell membrane, postsynaptic density, synapse, axon, and perikaryon.
• The protein undergoes post-translational modifications including palmitoylation (a lipoprotein modification) and phosphorylation at multiple sites; the Y340 residue is a phospho-acceptor site targeted by this antibody.
• Keywords: SH3 domain, PDZ domain repeat, alternative splicing, phosphoprotein.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around Y340. To confirm phospho-specificity, compare signals with and without phosphatase treatment or use a non-phosphopeptide blocking control.
• For Western blotting, include phosphatase inhibitors in the lysis buffer to preserve phosphorylation. A band near 98 kDa is expected for DLG2.
• The antibody is reported to react with human, mouse, and rat samples; validate in your specific cell or tissue system considering the expression profile of DLG2.
• For ELISA, the antibody can be used as a capture or detection reagent against the phospho-Y340 peptide; optimize coating and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines procurement for diagnostic manufacturers, laboratories, and research institutes by offering a comprehensive portfolio of IVD raw materials. For neuroscience and pain research targeting DLG2/PSD-93, our inventory includes validated antibody pairs, optimized polyclonal antibodies, and ancillary reagents. Our sourcing capabilities extend to rare-target raw materials, ensuring you have reliable access to the components needed for assay development from concept to clinic.