Phospho-PRMT4/CARM1 (Ser228) Rabbit Polyclonal Antibody for WB and ELISA - Q86X55

Core Product Specifications and Parameters

Biological Background: PRMT4/CARM1 Function and Localization

• As a coactivator-associated arginine methyltransferase, CARM1 methylates histone H3 at Arg-17 (H3R17me2a), facilitating chromatin remodeling and transcriptional activation. Related references: PMID:12237300 PMID:16497732 PMID:19405910
• Methylates EP300/P300 at Arg-2142, Arg-580, and Arg-604, modulating its interaction with NCOA2/GRIP1 and CREB, thereby affecting CREB-dependent transcription. Related references: PMID:15731352
• Independently of methyltransferase activity, CARM1 promotes PARP1 recruitment to replication forks, resulting in chromatin poly-ADP-ribosylation and reduced fork speed. Related references: PMID:33412112
• Acts as a transcriptional coactivator for nuclear hormone receptors, TCF/β-catenin, MEF2C, NF-κB, and PPARG pathways, supporting roles in adipogenesis and inflammatory responses (By similarity).
• Methylates splicing factors and RNA-binding proteins (e.g., PABPC1, ELAVL1, ELAV4), influencing alternative pre-mRNA splicing and mRNA stability (By similarity).
• Localizes to the nucleus, cytoplasm, and chromosomes, consistent with its dual roles in chromatin regulation and cytoplasmic mRNA metabolism.
• Overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia, indicating a potential role in cancer progression.

Experimental Guidance and Technical Tips

• Confirm phospho-specificity by peptide competition (phosphorylated vs. non-phosphorylated peptide) or treatment with protein phosphatase.
• Expected WB band: ~66 kDa; note possible additional bands from alternative splicing isoforms or cross-reactivity with other phosphorylated forms.
• For weak signals, consider stimulating cells to increase CARM1 Ser228 phosphorylation (e.g., with growth factors or hormones) before lysis.
• ELISA applications may require optimized coating concentrations and blocking conditions; validate with known positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw material procurement, offering validated antibodies, bulk ancillary reagents, and rare target sourcing for diagnostic and research workflows. This phospho-CARM1 antibody supports studies in transcriptional regulation and cancer, and we can assist with custom quantities, matched pairs, and technical consultation to advance your assay development.