Phospho-PPP1R12A/PPP1R12B/PPP1R12C (Thr696) Rabbit Polyclonal Antibody for WB, ELISA - O14974 / O60237 / Q9BZL4

Core Product Specifications and Parameters

Biological Background: PPP1R12A/PPP1R12B/PPP1R12C Function and Localization

• PPP1R12A (also known as MYPT1) is a key regulatory subunit of protein phosphatase 1 (PP1), targeting the catalytic subunit (PPP1C) to specific substrates.
• It mediates binding to myosin and regulates cytoskeletal dynamics, particularly in stress fibers, as part of the myosin phosphatase complex.
• The protein contains ANK repeats and is involved in dephosphorylation of PLK1, impacting cell cycle progression.
• PPP1R12A can inhibit HIF1AN-dependent suppression of HIF1A, thereby modulating hypoxic response pathways.
• Expression is prominent in striated muscles, especially type 2a fibers, indicating a role in muscle contraction regulation.
• The gene undergoes alternative splicing, producing functionally distinct isoforms.
• Keywords: Phosphoprotein, hydroxylation, and direct protein sequencing; highlighting post-translational modifications that regulate its activity.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide corresponding to residues surrounding Thr696 of human PPP1R12A. This site is conserved in PPP1R12B and PPP1R12C.
• For Western blot, include phosphatase inhibitors in lysis buffer to preserve phosphorylation status. Consider using phosphatase-treated lysate as a negative control.
• Validate antibody specificity in relevant human cell lines known to express these myosin phosphatase targets, such as HeLa or A431 cells.
• Phosphorylation at Thr696 may be induced by specific stimuli; optimizing stimulation conditions can enhance signal detection.
• For ELISA, use phosphopeptide antigen coating and compare with non-phosphorylated peptide to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for IVD raw material procurement, offering validated antibodies, bulk ancillary reagents, and customized sourcing for diagnostic development. For phosphorylation-sensitive targets like PPP1R12A/B/C, we supply rabbit polyclonal antibodies and can assist with matched antigen and recombinant protein needs. From research-grade materials to scalable IVD solutions, our team supports customers in achieving reliable assay performance.