Phospho-PPP1R12A-S668 Rabbit Polyclonal Antibody for WB, ELISA - O14974

Core Product Specifications and Parameters

Biological Background: PPP1R12A Function and Localization

• PPP1R12A, also known as MYPT1 (Myosin phosphatase-targeting subunit 1), is the regulatory subunit 12A of protein phosphatase 1 (PP1), a key Ser/Thr phosphatase.
• It functions as a scaffold that targets PP1 catalytic subunit (PPP1C) to myosin, enabling dephosphorylation of myosin regulatory light chains and regulation of actomyosin contractility.
• As part of the myosin phosphatase complex, PPP1R12A is involved in the dephosphorylation of PLK1, contributing to mitotic progression control.
• The protein is capable of inhibiting HIF1AN (factor inhibiting HIF-1α)-dependent suppression of HIF1A activity, potentially linking cytoskeletal signaling to hypoxia responses.
• Subcellular localization includes the cytoplasm and stress fibers, consistent with its role in actomyosin cytoskeleton dynamics.
• Expressed in striated muscles, specifically in type 2a fibers, highlighting its importance in muscle contractility.
• Post-translational modifications include phosphorylation at multiple sites (including S668) and hydroxylation, which modulate its activity and interactions.
• The gene PPP1R12A (MBS, MYPT1) maps to human chromosome 12 and produces alternative splice isoforms.

Experimental Guidance and Technical Tips

• Since the immunogen is a synthetic phosphorylated peptide around Ser668, the antibody is expected to specifically recognize PPP1R12A phosphorylated at this residue. Ensure phosphatase inhibitors are included in lysis buffers to preserve phosphorylation.
• For Western blot, a band at approximately 115 kDa is predicted. Consider loading 20–30 µg of cell or tissue lysate and using appropriate controls, such as phosphatase-treated samples, to confirm specificity.
• Species cross-reactivity is predicted for human, mouse, and rat based on sequence homology; however, validation in the relevant experimental system is recommended.
• The antibody may be suitable for ELISA; as with all phospho-specific antibodies, pair with a total protein antibody for normalization in semi-quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop sourcing bridge for IVD raw materials, supporting diagnostic manufacturers, laboratories, and research institutes from concept to clinic. Our portfolio includes validated phospho-specific antibodies like this anti-Phospho-PPP1R12A-S668 rabbit pAb, targeting myosin phosphatase signaling and cytoskeletal regulation. Additionally, we offer optimized antibody pairs, bulk monoclonal and polyclonal antibodies, ancillary reagents, and custom sourcing for rare targets—streamlining your development pipeline.