Phospho-PPP1CA-T320 Rabbit mAb for WB, IHC-P, ELISA - P62136

Core Product Specifications and Parameters

Biological Background: PPP1CA Function and Localization

• PPP1CA encodes the catalytic subunit of serine/threonine-protein phosphatase PP1-alpha, which associates with over 200 regulatory proteins to form substrate-specific holoenzymes that dephosphorylate hundreds of biological targets (PubMed:28216226, PubMed:30158517, PubMed:35768504). Related references: PMID:28216226 PMID:30158517 PMID:35768504
• PP1-alpha is essential for cell division, transcription elongation, glycogen metabolism, muscle contractility, and protein synthesis (PubMed:35768504, PubMed:35830882, PubMed:35831509). Related references: PMID:35768504 PMID:35830882 PMID:35831509
• As a catalytic component of the PNUTS-PP1 complex, PP1-alpha promotes RNA polymerase II transcription pause-release and termination, and is involved in chromatin structure and cell cycle progression during mitosis-interphase transition (PubMed:39603239, PubMed:39603240; PubMed:31677974; PubMed:20516061). Related references: PMID:39603239 PMID:39603240 PMID:31677974 PMID:20516061
• PP1-alpha functions in innate immunity by dephosphorylating IFIH1/MDA5 and RIG-I, thereby activating antiviral responses, and is necessary for alphavirus replication (PubMed:23499489). Related references: PMID:23499489
• PP1-alpha is a core component of the SHOC2-MRAS-PP1c (SMP) holoenzyme complex, which enhances its dephosphorylation activity and substrate specificity, particularly dephosphorylating RAF kinases to stimulate MAPK pathway activation (PubMed:35768504, PubMed:35830882, PubMed:35831509, PubMed:36175670). Related references: PMID:35768504 PMID:35830882 PMID:35831509 PMID:36175670
• Subcellularly, PP1-alpha localizes to the cytoplasm, nucleus (nucleoplasm and nucleolus), and postsynaptic density, consistent with its roles in diverse signaling pathways.

Experimental Guidance and Technical Tips

• This antibody was generated using a synthetic phospho-peptide around T320; phosphorylation site specificity should be confirmed by phosphatase treatment or competition with the non-phosphorylated peptide.
• For Western blot, the predicted molecular weight is approximately 37 kDa, but multiple bands (32 kDa, 37 kDa, 38 kDa) may be observed due to isoforms or post-translational modifications.
• Consider using appropriate positive and negative controls (e.g., phosphatase-treated lysates or cells stimulated to modulate PP1 activity) to validate detection of phosphorylated PPP1CA.
• For IHC-P, antigen retrieval and optimal antibody dilution should be empirically determined in the relevant tissue or cell sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to a comprehensive portfolio of IVD raw materials, including validated antibody pairs, high-performance monoclonal and polyclonal antibodies, and bulk ancillary reagents. Our expertise spans critical signaling targets such as protein phosphatases, enabling reliable sourcing for cell cycle, transcription, and cancer research applications. From concept to clinic, we support your development pipeline with consistent quality and custom sourcing solutions for rare and emerging targets.