Phospho-PP2A Catalytic α (Y307) Rabbit Monoclonal Antibody for WB, ELISA - P67775

Core Product Specifications and Parameters

Biological Background: PP2A Catalytic Subunit α Function and Localization

• PP2A catalytic subunit α (PPP2CA) is a serine/threonine phosphatase that regulates a broad array of enzymes and signaling pathways, including dephosphorylation of substrates such as p53/TP53, WEE1, and PIM3. Related references: PMID:10801873 PMID:12473674 PMID:17245430
• It is the major phosphatase for microtubule-associated proteins (MAPs) and modulates kinases such as phosphorylase B kinase, casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Related references: PMID:22613722
• PPP2CA dephosphorylates WEE1, preventing its degradation and promoting the G2/M checkpoint, and dephosphorylates MYC to enhance its ubiquitin-mediated proteolysis. Related references: PMID:33108758 PMID:25438055
• As part of the Integrator-PP2A (INTAC) complex, it mediates a quality checkpoint during transcription elongation by dephosphorylating the C-terminal domain of RNA polymerase II, triggering premature termination. Related references: PMID:33243860 PMID:34004147 PMID:37080207
• Subcellular localization is dynamic, with PPP2CA found in the cytoplasm, nucleus, centromere, and spindle pole, reflecting its diverse roles in mitosis and transcription.
• The protein undergoes post-translational modifications including phosphorylation at Tyr307 (the epitope recognized by this antibody), methylation, and binding of metal ions such as manganese and zinc.
• Alternative splicing generates multiple isoforms, and mutations in PPP2CA have been associated with intellectual disability.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phospho-peptide corresponding to Tyr307 of human PPP2CA. Verify phospho-specificity by treating samples with lambda phosphatase or by peptide competition using non-phosphorylated peptide.
• This antibody is indicated for Western blot (WB) and ELISA. For WB, the predicted molecular weight is 36 kDa. Include phosphatase inhibitors in lysis buffers to preserve the phosphorylated epitope.
• Since cross-reactivity with mouse is documented, validation in mouse-derived samples is advisable if used in that species. Confirm optimal dilution and detection conditions empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a complete range of IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This anti-phospho-PP2A catalytic α (Y307) monoclonal antibody supports research into phosphatase signaling, cell cycle regulation, and transcriptional control. Our one-stop platform simplifies procurement of rare target raw materials, helping you move from concept to clinic with confidence.