Phospho-POLR2A CTD-S2 Rabbit mAb - P24928

Core Product Specifications and Parameters

Biological Background: POLR2A Function and Localization

• POLR2A encodes the largest subunit of RNA polymerase II, which catalyzes transcription of mRNA precursors and many non-coding RNAs.
• Forms the active center with POLR2B, coordinating Mg2+ ions for nucleotide addition and proofreading via 3'->5' exonuclease activity. Related references: PMID:30190596 PMID:8381534
• Contains a unique C-terminal domain (CTD) with 52 heptapeptide repeats (YSPTSPS) that serves as a platform for factors regulating transcription initiation, elongation, and termination.
• CTD phosphorylation at Ser5 promotes promoter escape, while Ser2 phosphorylation is required for pause release and pre-mRNA processing. Related references: PMID:26124092
• Acetylation and methylation of CTD lysines regulate gene expression, with methylation having a repressive effect.
• Exhibits RNA-dependent RNA polymerase activity, extending non-coding RNAs at the 3' end.
• Subcellular localization includes nucleus, cytoplasm, and chromosome.
• Relevant keywords: phosphoprotein, acetylation, methylation, DNA-directed RNA polymerase, transcription, hydrolase.

Experimental Guidance and Technical Tips

• This phospho-specific antibody recognizes POLR2A only when Ser2 in the CTD repeat is phosphorylated. Include phosphatase inhibitors in lysis and wash buffers to preserve the phosphorylation signal.
• For chromatin immunoprecipitation (ChIP) and CUT&Tag, consider titrating the antibody and optimizing cross-linking/sonication conditions for your specific target loci.
• The antibody is suitable for Western blot (217 kDa), immunofluorescence, and immunohistochemistry; validate in the relevant sample system using appropriate positive and negative controls (e.g., untreated vs. kinase inhibitor-treated cells).
• Due to the high molecular weight, use a low-percentage SDS-PAGE gel or extended transfer times for WB.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with integrated access to high-quality IVD raw materials, technical services, and consulting. For transcription and RNA polymerase II research, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to support your assay development. Our one-stop sourcing model covers everything from initial concept to clinical application, including custom raw-material sourcing for rare or challenging targets.