Phospho-PLN-T17 Rabbit pAb - P26678

Core Product Specifications and Parameters

Biological Background: Phospholamban Function and Localization

• Phospholamban (PLN), also known as PLB, is a 52-amino acid transmembrane phosphoprotein predominantly expressed in cardiac muscle.
• It reversibly inhibits the sarcoplasmic reticulum Ca2+-ATPase SERCA2 (ATP2A2) by reducing its apparent affinity for Ca2+, thereby slowing Ca2+ re-uptake during muscle relaxation (PubMed:28890335). Related references: PMID:28890335
• PLN also modulates cardiac contractility in response to β-adrenergic stimulation; phosphorylation at Thr17 by CaMKII relieves SERCA2 inhibition, enhancing Ca2+ transport.
• Subcellularly, PLN localizes to the endoplasmic/sarcoplasmic reticulum membrane and also to the mitochondrial membrane, where it may influence calcium signaling and energy metabolism.
• PLN activity is regulated by its oligomeric state and post-translational modifications, including phosphorylation, acetylation, and palmitoylation, which affect its inhibitory function.
• Mutations in PLN are linked to dilated cardiomyopathy (CMD1P) and hypertrophic cardiomyopathy (CMH18), highlighting its critical role in cardiac function.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around Thr17; this antibody is expected to detect phospholamban only when phosphorylated at this site. Avoid using this antibody for detecting total PLN.
• For Western blot (WB), note the predicted molecular weight is 6 kDa; consider using a high-percentage gel (e.g., 12–15% Tris-tricine) and careful transfer conditions for small proteins.
• For immunohistochemistry (IHC-P), validate in cardiac muscle tissue where PLN is highly expressed; consider antigen retrieval methods appropriate for phospho-specific antibodies.
• For ELISA, the peptide-based immunogen suggests the antibody may work well in peptide competition assays; validate in the relevant sample system.
• Cross-reactivity is reported for human, mouse, and rat; for other species, check sequence homology around the phosphorylation site.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies this high-quality anti-phospho-PLN (Thr17) antibody alongside complementary IVD raw materials, including matched antibody pairs, recombinant proteins, and ancillary reagents for cardiac biomarker research. With end-to-end support from concept to clinic, we help diagnostic developers accelerate assay development for cardiac and phosphorylated protein targets. Contact our team for bulk orders or customized sourcing solutions.