Phospho-PLN-S16/T17 Rabbit Polyclonal Antibody for WB, ELISA - P26678

Core Product Specifications and Parameters

Biological Background: Phospholamban Function and Localization

• Phospholamban (PLN) is a key regulator of cardiac muscle contractility, encoded by the PLN gene, with a molecular weight of approximately 6 kDa (UniProt: P26678).
• It reversibly inhibits the sarco/endoplasmic reticulum Ca2+-ATPase 2 (SERCA2/ATP2A2) in cardiac sarcoplasmic reticulum, lowering the apparent Ca2+ affinity of the pump, particularly during diastole. Related references: PMID:28890335
• PLN phosphorylation at Ser16 and Thr17 relieves SERCA2 inhibition, promoting Ca2+ re-uptake into the sarcoplasmic reticulum and accelerating muscle relaxation.
• Subcellularly, PLN localizes to the endoplasmic reticulum, sarcoplasmic reticulum, and mitochondrial membranes, consistent with its roles in intracellular Ca2+ handling.
• Post-translational modifications include phosphorylation (at Ser16/Thr17), acetylation, and palmitoylation, which modulate its activity and membrane association.
• PLN is predominantly expressed in heart muscle, but it also plays roles in elongated spermatids and in the brain's thalamic reticular nucleus, influencing germ cell differentiation and sleep patterns, respectively.
• Mutations in PLN are associated with cardiomyopathies (CMD1P, CMH18), highlighting its critical role in cardiac function.

Experimental Guidance and Technical Tips

• Since the immunogen is a synthetic phospho-peptide spanning Ser16 and Thr17, the antibody is expected to specifically recognize the dual-phosphorylated form of PLN; validate specificity using dephosphorylated controls or phosphatase treatment in relevant sample systems.
• For Western blot analysis, use low molecular weight markers and consider 15-20% Tris-Tricine gels to resolve the 6 kDa PLN band; include phosphatase inhibitors during sample preparation to preserve phosphorylation.
• For ELISA, coating with phospho-peptide conjugates and using competitive or blocking peptides can help confirm phospho-specificity.
• Optimize antibody dilution empirically for each application; inclusion of appropriate positive and negative controls is recommended to confirm phospho-dependent reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents to streamline diagnostic development. With this phospho-PLN antibody targeting cardiac calcium signaling, we support research in cardiovascular disease and drug discovery. Our team also assists with rare target raw-material sourcing, providing end-to-end support from concept to clinic.