Phospho-PLN-S16 Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P26678

Core Product Specifications and Parameters

Biological Background: Phospholamban (PLN) Function and Localization

• Phospholamban (PLN; also known as PLB, CMD1P, CMH18) is a 6 kDa transmembrane protein that forms homopentamers in the sarcoplasmic reticulum membrane.
• It reversibly inhibits the cardiac Ca2+ ATPase SERCA2 by decreasing its apparent affinity for Ca2+, thereby regulating cardiac muscle relaxation and contractility (PubMed:28890335). Related references: PMID:28890335
• PLN binds preferentially to the ATP-bound E1 conformational state of SERCA2, which predominates at low cytosolic Ca2+ concentrations during diastole (By similarity).
• The inhibitory effect depends on PLN's oligomeric state and is relieved by phosphorylation; Ser16 is a key phosphorylation site that modulates SERCA2 activity.
• Beyond cardiac muscle, PLN also inhibits SERCA3 and controls intracellular Ca2+ levels in elongated spermatids, with a potential role in germ cell differentiation (By similarity).
• PLN localizes to endoplasmic reticulum, sarcoplasmic reticulum, and mitochondrial membranes, with strong expression in heart muscle.
• Keywords associated with PLN include cardiomyopathy, disease variant, phosphoprotein, acetylation, and palmitate, reflecting its critical role in cardiac function and regulation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide around Ser16 of human PLN. This ensures specificity for the phosphorylated form; validate with phosphatase treatment or non-phospho controls.
• For WB, a band around 6 kDa is expected (monomer) but may appear as pentamer depending on boiling conditions. Use low molecular weight markers and high-percentage gels.
• PLN phosphorylation at Ser16 is a key readout for β-adrenergic signaling in cardiac tissue. Include fresh or flash-frozen heart tissue from human, mouse, or rat.
• For IF-P and IHC-P, optimize fixation and antigen retrieval; phospho‑specific antibodies may require gentle fixation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. The Phospho-PLN-S16 Rabbit mAb supports cardiovascular disease research and assay development, enabling reliable detection of PLN phosphorylation. Beyond standard catalog products, CamelBio offers customized sourcing for rare target antibodies and raw materials, helping you accelerate your diagnostic pipeline from concept to clinic.