Phospho-PLC gamma 2 (PLCG2)-Y1217 Rabbit pAb - P16885

Core Product Specifications and Parameters

Biological Background: PLC gamma 2 (PLCG2) Function and Localization

• PLCG2 encodes 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2, also known as PLC-gamma-2 or PLC-IV.
• Alternative names include FCAS3 and APLAID, reflecting associations with autoinflammatory diseases.
• It catalyzes the production of second messengers diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3), crucial in transmembrane signaling (PubMed:19394299, PubMed:23000145). Related references: PMID:19394299 PMID:23000145
• Localizes to membrane rafts, where it participates in signal transduction.
• Contains multiple phosphorylation sites, including Y1217, which is critical for its activation.
• Annotated with keywords: 3D-structure, calcium, disease variant, hydrolase, lipid degradation, lipid metabolism, membrane, phosphoprotein, proteomics identification, reference proteome, repeat, SH2 domain, SH3 domain, transducer.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around Y1217 of human PLCG2. Treat samples with phosphatase inhibitors to preserve phosphorylation.
• For Western blotting, the predicted molecular weight of PLCG2 is ~148 kDa; optimize transfer conditions for high-molecular-weight proteins.
• For immunohistochemistry (IHC-P), consider antigen retrieval and antibody dilution optimization, and validate phospho-specificity in your system.
• Validate antibody reactivity in human and mouse samples, as cross-reactivity may require empirical confirmation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop sourcing solution for IVD raw materials, supporting diagnostic manufacturers and research laboratories. For studies on PLCG2 signaling and autoinflammatory diseases, we can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Our team also assists in sourcing rare target raw materials to accelerate your workflow.