Phospho-PLC gamma 1 (PLCG1)-Y783 Rabbit pAb for WB, ELISA - P19174

Core Product Specifications and Parameters

Biological Background: Phospho-PLC gamma 1 (PLCG1)-Y783 Function and Localization

• PLCG1 encodes 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1, a key enzyme in phosphoinositide signaling. It hydrolyzes PIP2 to generate the second messengers DAG and IP3, which mobilize calcium and activate protein kinase C.
• It is activated downstream of receptor tyrosine kinases (e.g., PDGFRA, PDGFRB, EGFR, FGFRs), playing a critical role in transducing growth factor signals to intracellular pathways.
• PLCG1 participates in actin reorganization and cell migration, as shown by its requirement for efficient chemotaxis (PubMed:17229814). Related references: PMID:17229814
• The protein localizes to lamellipodia and membrane ruffles, dynamic structures at the leading edge of migrating cells, underscoring its role in cytoskeletal regulation.
• PLCG1 contains SH2 and SH3 domains that mediate interactions with phosphorylated tyrosine residues on activated receptors, and itself is phosphorylated at key tyrosine residues (including Y783) for full activation.
• It is subject to various post-translational modifications, including phosphorylation and acetylation, and is classified as a phosphoprotein, hydrolase, and transducer.

Experimental Guidance and Technical Tips

• This antibody is raised against a phosphorylated peptide; therefore, it specifically recognizes PLCG1 phosphorylated at Y783. For negative controls, treat samples with lambda phosphatase or use non-phosphorylated peptide competition to confirm specificity.
• In Western blot, the predicted molecular weight of PLCG1 is ~149 kDa. Ensure adequate separation of high-molecular-weight proteins using appropriate gel percentage (e.g., 6-8% SDS-PAGE) and transfer conditions.
• For ELISA, the antibody can be used as a detection antibody in a phospho-specific sandwich ELISA format. Validate in the relevant sample system, such as stimulated vs. unstimulated cell lysates.
• When detecting endogenous PLCG1 phosphorylation, consider stimulating cells with growth factors (e.g., EGF, PDGF) known to activate receptor tyrosine kinases that phosphorylate PLCG1 at Y783.
• Cross-reactivity with mouse is indicated; however, confirm reactivity in your specific experimental model and validate the antibody for other species if needed.
• Since the antibody is a rabbit polyclonal, batch-to-batch variability might occur; include appropriate controls and titrate for optimal concentration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with one-stop access to high-quality IVD raw materials, technical services, and consulting—from concept to clinic. For studies on PLCG1 signaling and tyrosine phosphorylation, we offer this phospho-specific antibody along with a wide portfolio of phosphorylation-related antibodies, bulk reagents, and custom sourcing solutions to streamline your assay development.