Phospho-PLC gamma 1 (PLCG1)-S1248 Rabbit pAb - P19174

Core Product Specifications and Parameters

Biological Background: PLC gamma 1 Function and Localization

• Encoded by the PLCG1 gene, this protein is also known as 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1, PLC-148, PLC-II, and PLC-gamma-1; it belongs to the phospholipase C family.
• As a critical signal transducer, it hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to generate the second messengers inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG), driving calcium mobilization and protein kinase C activation.
• PLC gamma 1 is activated downstream of receptor tyrosine kinases (e.g., PDGFRA, EGFR, FGFRs) via phosphorylation at key tyrosine residues and mediates cellular responses such as proliferation, differentiation, and survival.
• It plays a role in actin reorganization and cell migration, contributing to lamellipodium and ruffle dynamics. Related references: PMID:17229814
• An additional function is its guanine nucleotide exchange factor (GEF) activity toward dynamin-1 (DNM1), enhancing endocytosis by catalyzing GDP/GTP exchange.
• Subcellularly, PLC gamma 1 localizes to the cell periphery, including lamellipodia and ruffles, consistent with roles in cell motility and membrane trafficking.
• The protein is subject to extensive post-translational modifications, including phosphorylation (e.g., at S1248), acetylation, and ubiquitination, which regulate its activity and interactions.
• UniProtKB keywords highlight its involvement in host-virus interactions and disease variants, and it is classified under lipid degradation, metal-binding (calcium), and SH2/SH3 domain-containing signaling proteins.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide matching human PLCG1 S1248; confirm antibody specificity by comparing signals in phosphatase-treated versus untreated samples.
• For Western blotting, use a membrane blocking buffer compatible with phospho-antibodies and include phosphatase inhibitors in lysis buffers to preserve phosphorylation.
• In immunohistochemistry (paraffin), consider antigen retrieval optimization; positive staining in tissues with active receptor tyrosine kinase signaling (e.g., tumor sections) may serve as biological control.
• When performing ELISA, validate the coating antigen and detection range using a recombinant phospho-peptide standard if available; cross-reactivity with mouse and rat PLCG1 allows multispecies study designs.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies this phospho-PLCG1 antibody to researchers and diagnostic developers studying phospholipase C signaling and tyrosine kinase pathways. As a one-stop IVD raw material partner, we offer validated antibody pairs, bulk monoclonal/polyclonal antibodies, ancillary reagents, and custom sourcing for rare targets—supporting your projects from concept to clinic. Contact us to discuss procurement optimization and technical consultation for your specific assay development needs.