Phospho-PLC gamma 1 (PLCG1)-S1248 Rabbit Monoclonal Antibody for WB, ELISA - P19174

Core Product Specifications and Parameters

Biological Background: PLCG1 Function and Localization

• PLCG1 encodes 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1, also known as PLC-gamma-1, PLC-II, and PLC148, and is expressed in human cells as a 149 kDa protein (SWISS: P19174).
• It catalyzes the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) to generate the second messengers diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3), linking receptor tyrosine kinase activation to intracellular calcium release and PKC activation (By similarity).
• PLCG1 is activated by ligand-bound receptor tyrosine kinases including PDGFRA, PDGFRB, EGFR, FGFR1, FGFR2, FGFR3, and FGFR4, playing a pivotal role in growth factor signal transduction (By similarity).
• It is involved in actin cytoskeleton reorganization and cell migration, contributing to lamellipodium and ruffle formation (PubMed:17229814). Related references: PMID:17229814
• PLCG1 also functions as a guanine nucleotide exchange factor (GEF) for dynamin-1 (DNM1), facilitating GDP dissociation and enhancing DNM1-dependent endocytosis (By similarity).
• The protein contains SH2 and SH3 domains, which mediate interactions with phosphorylated tyrosine residues on activated receptors and downstream effectors, and is subject to phosphorylation, acetylation, and ubiquitin-like conjugation.
• Subcellularly, PLCG1 localizes to the cytosol and is recruited to the plasma membrane upon activation, with enrichment at cell projections such as lamellipodia and ruffles.
• Alternative splicing produces multiple isoforms, and genetic variants in PLCG1 are associated with diseases such as immune dysregulation and cancer.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a phosphorylated peptide around serine 1248 of human PLCG1. Consider verifying phosphorylation status in your sample system using phosphatase treatment controls.
• For western blotting, the predicted molecular weight is approximately 149 kDa; use appropriate gel percentage and transfer conditions to resolve this high-molecular-weight protein.
• Cross-reactivity has been reported with human and mouse PLCG1; validate detection in other species or isoform-specific contexts as needed.
• In ELISA, optimize coating antigen and detection conditions to ensure specificity for the phosphorylated epitope.
• Given PLCG1's role in signal transduction, stimulate cells with growth factors (e.g., EGF, PDGF) to enhance phosphorylation at S1248 prior to analysis.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to premium IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. With expertise in phospho-specific detection and signal transduction research, we support your assay development for targets like PLCG1, offering consistent quality and reliable supply from concept to clinic. Contact our team for custom sourcing or technical consultation on phosphorylated antibodies and rare target raw materials.